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Updated: Feb 27, 2026
Microfluidic Chip Fabrication and Method to Detect Influenza
Published on: March 26, 2013
An integrated versatile lab-on-a-chip platform for the isolation and nucleic acid-based detection of pathogens
Natalia Sandetskaya1,1, Doreen Moos1,1, Harald Pötter2,2
1Fraunhofer Institute for Cell Therapy & Immunology, Leipzig, 04103, Germany.
Aim:
Processing of the samples in molecular diagnostics is complex and labor-intensive. An integrated and automated platform for sample preparation and nucleic acid-based detection can significantly relieve this burden for the users.
Results:
We present a prototype of a versatile and integrated platform for the detection of pathogens in various liquid media. We describe a proof-of-concept for the integrated isolation of bacteria, cell lysis with optional DNA extraction, DNA amplification and detection in two different reactions, loop-mediated isothermal amplification and PCR, on a single microfluidic platform.
Conclusion:
The platform enables the transition from large sample volume to microfluidic format. The design and open interface enable its versatile application for various nucleic acid-based assays, from simple to complex setups.
