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Updated: Feb 27, 2026

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
Published on: March 28, 2025
Maize hybrid stability in environments under water restriction using mixed models and factor analysis.
A O Santos1, J J Nuvunga2, C P Silva2
1Departamento de Agricultura, Universidade Federal de Lavras, Lavras, MG, Brasil.
Maize hybrids G66, G99, G86, and G26 demonstrate superior phenotypic stability in both water-restricted and non-restricted environments. The analytical factor approach effectively identifies adaptable and stable hybrids for high-yield crop production.
Area of Science:
- Agricultural Science
- Plant Breeding
- Agronomy
Background:
- Water deficit is a primary constraint on crop yield, particularly in maize production.
- Evaluating phenotypic stability is crucial for developing resilient crop varieties.
- The analytical factor (AF) approach offers a novel method for assessing hybrid performance across diverse conditions.
Purpose of the Study:
- To assess the phenotypic stability of 171 maize hybrids under varying water availability conditions.
- To identify maize hybrids exhibiting consistent grain yield and desirable agronomic traits.
- To validate the utility of the analytical factor (AF) approach in multi-environment trials.
Main Methods:
- Evaluation of 171 maize hybrids across 14 environments over 7 years, with distinct water-restricted and non-restricted zones.
- Utilized a square lattice design (6 x 6) with common treatments across years for robust statistical analysis.
- Assessed key agronomic traits including grain yield (GY), male flowering (MF), female flowering (FF), plant height (PH), and ear height (EH).
Main Results:
- Hybrids G66, G99, G86, and G26 were identified as the most stable performers.
- These top-performing hybrids demonstrated significant potential for cultivation in both water-stressed and optimal environments.
- The AF models proved effective in correlating hybrid performance with production components and identifying stable mega-environments.
Conclusions:
- The analytical factor (AF) approach is a valuable tool for evaluating long-term hybrid performance and stability.
- Selected hybrids (G66, G99, G86, G26) offer promising solutions for enhancing maize production under water deficit stress.
- The study underscores the importance of phenotypic stability for developing climate-resilient maize varieties.
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