Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

16.6K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
16.6K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

17.3K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
17.3K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

7.7K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Secreted IgM deficiency alters the retinal landscape enhancing neurodegeneration associated with aging.

Immunity & ageing : I & A·2025
Same author

Complete primer set for amplification and expression of full-length recombinant human monoclonal antibodies from single human B cells.

Journal of immunological methods·2025
Same author

An optimized filter trap assay for detecting recombinant authentic tau fibrils.

Free neuropathology·2024
Same author

A novel approach to Parkinson's disease treatment with a potentially dual-acting therapeutic agent that targets α-synuclein aggregation and neuron death.

Neural regeneration research·2024
Same author

TLR Engagement Induces an Alternate Pathway for BCR Signaling that Results in PKCδ Phosphorylation.

Journal of immunology (Baltimore, Md. : 1950)·2024
Same author

Human VH4-34 antibodies derived from B1 cells are more frequently autoreactive than VH4-34 antibodies derived from memory cells.

Frontiers in immunology·2024

Related Experiment Video

Updated: Feb 27, 2026

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
08:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Published on: May 10, 2022

2.7K

CD25 B-1a Cells Express Aicda.

Hiroaki Kaku1, Nichol E Holodick1, Joseph R Tumang2

  • 1Center for Oncology and Cell Biology, The Feinstein Institute for Medical Research, Manhasset, NY, United States.

Frontiers in Immunology
|July 6, 2017
PubMed
Summary

Activation-induced cytidine deaminase (AID) is expressed in B-1a cells, specifically within the CD25+ subpopulation. AID deficiency enhances B-1a cell progenitor vigor in competitive settings, suggesting AID inhibits B-1a cell development.

Keywords:
AIDB-1 cell subsetB-1a cellsCD25peritoneal cavity

More Related Videos

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
07:18

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

Published on: April 11, 2011

15.9K
An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

16.0K

Related Experiment Videos

Last Updated: Feb 27, 2026

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
08:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Published on: May 10, 2022

2.7K
HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
07:18

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

Published on: April 11, 2011

15.9K
An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

16.0K

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • B-1a cells are innate-like lymphocytes that produce natural antibodies.
  • Activation-induced cytidine deaminase (AID), encoded by the Aicda gene, is crucial for B cell class-switch recombination and somatic hypermutation.
  • Previous studies hinted at Aicda's role in B-1a cells using knockout mice, but direct expression and subpopulation specificity were unconfirmed.

Purpose of the Study:

  • To determine if B-1a cells express Aicda.
  • To identify which B-1a cell subpopulations express Aicda.
  • To investigate the functional role of AID in B-1a cell development and progenitor competition.

Main Methods:

  • Quantitative analysis of Aicda gene expression in B-1a cells and comparison with germinal center (GC) B cells.
  • Subpopulation analysis of Aicda expression within B-1a cells based on CD25 marker.
  • Competitive bone marrow reconstitution assays using AID knockout (KO) and wild-type (WT) B-1 cell progenitors.

Main Results:

  • B-1a cells express Aicda, albeit at lower levels than GC B cells.
  • Aicda expression in B-1a cells is predominantly found in the CD25+ subpopulation.
  • B-1a cell progenitors lacking AID (AID KO) exhibited enhanced competitive vigor compared to WT progenitors in bone marrow reconstitution assays.

Conclusions:

  • These findings identify CD25+ B-1a cells as a distinct subpopulation expressing Aicda, potentially impacting previous memory B cell studies.
  • The results suggest that AID actively inhibits the development of B-1a cells from bone marrow progenitors within a competitive environment.
  • This study elucidates a novel regulatory role for AID in B-1a cell lineage development.