Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Evidence for G6PD variant classification from multiplexed functional assays.

Genome biology·2026

Same author

Microbial Differences in Prostate Lesions Between Black and White American Men.

The Prostate·2026

Same author

A cross-species rescue by mating method to interrogate gene essentiality across the <i>Saccharomyces</i> genus.

microPublication biology·2026

Same author

Beyond ERCs: exploring catastrophic forms of rDNA instability in aging yeast.

bioRxiv : the preprint server for biology·2026

Same author

Telomeric amplicons of <i>SUL1</i> and Y' in yeast are generated by microhomology-mediated break induced replication occurring <i>in cis</i>.

bioRxiv : the preprint server for biology·2026

Same author

Using Experimental Evolution to Correct Mother-Daughter Separation Defects in Brewing Yeast.

microPublication biology·2026

Search research articles

Related Experiment Video

Updated: Feb 27, 2026

Design and Use of Multiplexed Chemostat Arrays

Design and Use of Multiplexed Chemostat Arrays

Published on: February 23, 2013

Assembly of a Mini-Chemostat Array.

Aaron W Miller¹, Emily O Kerr¹, Maitreya J Dunham²

¹Department of Genome Sciences, University of Washington, Seattle, Washington 98195.

Cold Spring Harbor Protocols

|July 7, 2017

Summary

Researchers can now access continuous culture growth with inexpensive, high-throughput miniature chemostats (ministats). These devices enable reproducible studies of yeast physiology, gene expression, and evolution, making advanced research more accessible.

More Related Videos

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Published on: June 6, 2017

Fabrication and Use of MicroEnvironment microArrays MEArrays

Fabrication and Use of MicroEnvironment microArrays MEArrays

Published on: October 11, 2012

Related Experiment Videos

Last Updated: Feb 27, 2026

Design and Use of Multiplexed Chemostat Arrays

Design and Use of Multiplexed Chemostat Arrays

Published on: February 23, 2013

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Published on: June 6, 2017

Fabrication and Use of MicroEnvironment microArrays MEArrays

Fabrication and Use of MicroEnvironment microArrays MEArrays

Published on: October 11, 2012

Area of Science:

Microbiology
Biotechnology
Evolutionary Biology

Background:

Continuous culture is vital for studying yeast physiology, strain competition, and long-term evolution.
Commercial platforms for continuous culture can be expensive and less accessible for high-throughput studies.

Purpose of the Study:

To describe the assembly of inexpensive, miniature chemostats (ministats) for continuous culture experiments.
To demonstrate the reproducibility and utility of these ministats for yeast research.

Main Methods:

Assembly of miniature (20-mL) chemostats using off-the-shelf components.
Conducting yeast culture experiments in ministats and comparing results with commercial platforms.

Main Results:

Demonstrated reproducibility in cellular physiology, gene expression, and evolutionary outcomes across different ministats.
Observed comparable results between ministats and larger commercial continuous culture platforms.

Conclusions:

Inexpensive ministats provide a accessible and high-throughput method for continuous culture growth.
These devices facilitate reproducible research in yeast physiology, gene expression, and evolution.