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Validation of rapid algal bioassay using delayed fluorescence in an interlaboratory ring study
Masakazu Katsumata1, Yuko Ikushima1, Keith Bennett1
1Central Research Laboratory, Hamamatsu Photonics K.K., Japan.
The Science of the Total Environment
|July 7, 2017
Summary
This study introduces a rapid delayed fluorescence (DF) test to assess chemical toxicity in algae, offering a faster alternative to traditional growth inhibition tests. The DF method effectively measures algal biomass, providing reliable toxicity data in just 24 hours.
Area of Science:
- Environmental Toxicology
- Algal Assays
- Biomass Measurement
Background:
- Algal growth inhibition tests are standard for evaluating chemical toxicity.
- Conventional tests require 72-96 hours for results.
- There is a need for faster, reliable toxicity assessment methods.
Purpose of the Study:
- To develop and validate a rapid algal toxicity test using delayed fluorescence (DF).
- To assess DF as an alternative endpoint for algal biomass production.
- To determine the variability of the DF method through an interlaboratory study.
Main Methods:
- Utilized delayed fluorescence (DF) measurements as a proxy for algal biomass.
- Conducted toxicity tests with 3,5-dichlorophenol (DCP) using DF.
- Performed an interlaboratory ring study to assess method variability.
Main Results:
- DF measurements correlated well with photosynthetically-active biomass.
- EC50 values from 6h and 24h DF tests showed agreement with conventional 72h tests.
- Interlaboratory variability for the 24h DF test was 12% (intralaboratory) and 28% (interlaboratory).
Conclusions:
- Delayed fluorescence (DF) shows potential as a surrogate for algal biomass in toxicity testing.
- A 24-hour DF exposure period provides reliable toxicity estimates comparable to conventional methods.
- The DF method offers a rapid and robust alternative for algal toxicity assessments.
