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Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy
Katarína Rebrošová1, Martin Šiler2, Ota Samek2
1Department of Microbiology, Faculty of Medicine & St Anne's Faculty Hospital, Pekařská 53, Brno 65691, Czech Republic.
Aim:
Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid noninvasive technique.
Materials & Methods:
For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy.
Discussion & Conclusion:
The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.
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