Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

9.3K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
9.3K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K
Mass Spectrometers01:16

Mass Spectrometers

9.4K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
9.4K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.7K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.7K
Mass Spectrometry: Molecular Fragmentation Overview01:20

Mass Spectrometry: Molecular Fragmentation Overview

5.9K
The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can occur at...
5.9K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.2K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Navigating Challenges in Mass Spectrometry Analysis of Endogenous and Synthetic Protein Modifications.

Biomolecules·2026
Same author

Inhibition of USP7 Destabilizes the Noncanonical PRC1.1 Complex and Induces Neuroblastoma Differentiation.

Molecular cancer research : MCR·2026
Same author

Overcoming EGFR resistance by monovalent and bident inhibitors targeting Cys775.

bioRxiv : the preprint server for biology·2026
Same author

Structure-Guided Design of Potent and Selective Covalent Inhibitors Targeting the SARS-CoV-2 Papain-like Protease.

Journal of medicinal chemistry·2026
Same author

CoREST complex inhibition alters RNA splicing to promote neoantigen expression and enhance tumor immunity.

JCI insight·2025
Same author

Structural basis for the recruitment and selective phosphorylation of Akt by mTORC2.

Science (New York, N.Y.)·2025

Related Experiment Video

Updated: Feb 27, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

1.1K

multiplierz v2.0: A Python-based ecosystem for shared access and analysis of native mass spectrometry data.

William M Alexander1,2, Scott B Ficarro1,2, Guillaume Adelmant1,2

  • 1Department of Cancer Biology and Blais Proteomics Center, Dana-Farber Cancer Institute, Boston, MA, USA.

Proteomics
|July 8, 2017
PubMed
Summary

This study introduces an updated Python software library, multiplierz, and its graphical interface, mzDesktop, to improve proteomic data analysis and distribution. These tools enhance mass spectrometry data accessibility and processing for biological research.

Keywords:
Application programming interface (API)Python

More Related Videos

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

13.5K
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
06:43

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

9.2K

Related Experiment Videos

Last Updated: Feb 27, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

1.1K
Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

13.5K
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
06:43

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

9.2K

Area of Science:

  • Proteomics
  • Bioinformatics
  • Computational Biology

Background:

  • Modern mass spectrometry generates large, diverse datasets challenging data access and analysis.
  • Understanding molecular mechanisms in physiology and disease requires robust proteomic data handling.

Purpose of the Study:

  • To present a redesigned version of the multiplierz Python software library and its associated mzDesktop graphical user interface.
  • To enhance the analysis and distribution of proteomic data from mass spectrometry experiments.

Main Methods:

  • Redesigned multiplierz Python library with mzAPI for data analysis.
  • Integration of new features: broader native file type support, additional database search engine interfaces, new reporting formats.
  • Development of mzDesktop, a GUI for user-friendly access to multiplierz functionality.
  • Implementation of high-level tools for post-search proteomic analyses.

Main Results:

  • Multiplierz now supports a wider array of mass spectrometry file types.
  • Enhanced compatibility with various database search engines and reporting formats.
  • Introduction of mzDesktop for simplified user interaction with advanced proteomic analysis tools.

Conclusions:

  • The redesigned multiplierz and mzDesktop facilitate improved community-wide access, analysis, and distribution of proteomic data.
  • These tools address challenges posed by the increasing scale and diversity of mass spectrometry data.
  • Enhanced proteomic data processing aids in deciphering molecular mechanisms of health and disease.