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Design and Analysis of Temperature Preference Behavior and its Circadian Rhythm in Drosophila
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Body Temperature Cycles Control Rhythmic Alternative Splicing in Mammals.

Marco Preußner1, Gesine Goldammer1, Alexander Neumann1

  • 1Laboratory of RNA Biochemistry, Freie Universität Berlin, Institute of Chemistry and Biochemistry, Takustrasse 6, 14195 Berlin, Germany.

Molecular Cell
|July 11, 2017
PubMed
Summary
This summary is machine-generated.

Mammalian body temperature cycles regulate gene activity through alternative splicing (AS). A slight 1°C shift triggers coordinated AS changes, impacting TATA-box binding protein (TBP) levels and gene expression, independent of core clock mechanisms.

Keywords:
SR proteinsTbpU2afalternative splicingbody temperaturecircadian clockmolecular thermometerphosphorylationsplicing network

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Area of Science:

  • Molecular Biology
  • Chronobiology
  • Genetics

Background:

  • Mammalian core body temperature exhibits daily oscillations.
  • The molecular mechanisms linking physiological temperature changes to cellular processes are not fully understood.

Purpose of the Study:

  • To investigate the molecular consequences of physiological body temperature fluctuations.
  • To determine if body temperature cycles influence gene expression through alternative splicing.

Main Methods:

  • Analysis of SR protein phosphorylation patterns in response to temperature changes.
  • Assessment of alternative splicing (AS) events in functionally related genes.
  • Examination of TATA-box binding protein (Tbp) alternative splicing and its effect on TBP protein levels.

Main Results:

  • Body temperature cycles were found to drive rhythmic SR protein phosphorylation, controlling an alternative splicing program.
  • A 1°C temperature change induced a coordinated splicing switch in numerous genes, demonstrating a sensitive temperature-sensing mechanism.
  • Alternative splicing of two exons in the TBP 5' UTR led to rhythmic TBP protein levels, affecting global gene expression in vivo.

Conclusions:

  • Body temperature-driven alternative splicing acts as a sensitive, clock-independent oscillator in mammalian peripheral clocks.
  • This mechanism provides a novel link between physiological temperature rhythms and gene regulation.