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Related Experiment Video

Updated: Feb 27, 2026

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
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Recent advances in high-throughput approaches to dissect enhancer function.

David Santiago-Algarra1, Lan T M Dao1, Lydie Pradel1

  • 1Aix-Marseille University, TAGC, Marseille, France.

F1000Research
|July 11, 2017
PubMed
Summary

Enhancer elements are crucial for gene transcription regulation, development, and cell differentiation. Recent high-throughput methods, including CRISPR/Cas9, advance the study of enhancer activity and its role in human disease.

Keywords:
CRISPRMPRASTARR-seqenhancer functiongene transcription

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Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Gene transcription regulation in eukaryotes involves promoters and enhancers.
  • Enhancers are critical for development and cell differentiation.
  • Genetic variations in enhancers are linked to human diseases.

Purpose of the Study:

  • To review recent high-throughput technologies for assessing enhancer activity.
  • To highlight advancements in understanding enhancer function and regulatory roles.
  • To discuss the impact of enhancer alterations on transcriptional regulation.

Main Methods:

  • Massively parallel reporter assays (MPRAs).
  • Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based technologies.
  • High-throughput screening approaches for enhancer identification and characterization.

Main Results:

  • Recent technologies offer powerful tools to measure enhancer activity at scale.
  • These methods facilitate the discovery of novel regulatory sequences.
  • Understanding enhancer function is advancing rapidly.

Conclusions:

  • High-throughput approaches are revolutionizing enhancer research.
  • Enhanced understanding of enhancers improves insights into development and disease.
  • CRISPR/Cas9 and MPRAs are key technologies for dissecting enhancer roles in gene regulation.