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Updated: Feb 26, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Immunoassay quantification using surface-enhanced fluorescence (SEF) tags.

S A Camacho¹, R G Sobral-Filho², P H B Aoki³

¹São Paulo State University (UNESP), School of Technology and Applied Sciences, Campus Presidente Prudente, SP, Brazil19060-900 and University of Victoria, Department of Chemistry, Victoria, BC, CanadaV8P 5C2. agbrolo@uvic.ca.

|July 14, 2017

Summary

New surface-enhanced fluorescence (SEF) tags using gold nanoparticles offer a sensitive method for detecting Immunoglobulin-M (IgM). These novel SEF tags enable quantification of IgM down to 10 ng/mL, improving diagnostic capabilities.

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Area of Science:

Biomedical research
Nanotechnology
Analytical chemistry

Background:

Fluorescence immunoassays are crucial for biomedical research and diagnostics.
Existing organic dye probes have limitations, necessitating development of new fluorescence tags.
There is a need for highly sensitive fluorescence tags with low limits of detection.

Purpose of the Study:

To explore the surface-enhanced fluorescence (SEF) phenomenon for developing novel tags.
To create SEF tags for sensitive detection and quantification of Immunoglobulin-M (IgM).

Main Methods:

Synthesis of shell-isolated gold nanoparticles (Au-SHINs) with a Nile Blue fluorescent layer.
Coating Au-SHINs with silica to create SEF tags.
Functionalization of SEF tags with antibodies for IgM detection in a sandwich immunoassay format.

Main Results:

Successful detection of IgM concentrations as low as 10 ng/mL.
Demonstrated a linear correlation between fluorescence intensity and IgM concentration.
Developed a novel SEF tag system for enhanced fluorescence detection.

Conclusions:

The developed SEF tags show significant potential for sensitive IgM detection.
This approach offers an improvement over traditional fluorescence-based immunoassay probes.
The SEF tags can be utilized in various diagnostic and research applications requiring high sensitivity.