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Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
Published on: February 22, 2019
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Genome wide host gene expression analysis in mice experimentally infected with Pasteurella multocida
G Bhuvana Priya1, Viswas Konasagara Nagaleekar1, A Arun Prince Milton2
1Division of Bacteriology & Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
Plos One
|July 14, 2017
Summary
This study reveals how the bacterium Pasteurella multocida triggers host immune responses in mice. Gene expression analysis identified key signaling pathways involved in the host
Area of Science:
- Veterinary Microbiology
- Immunology
- Genomics
Background:
- Pasteurella multocida causes fatal hemorrhagic septicemia in cattle and buffalo.
- Understanding host-pathogen interactions is crucial for disease control.
Purpose of the Study:
- To investigate host gene expression profiles during P. multocida infection in mice.
- To identify key immune signaling pathways activated by P. multocida.
Main Methods:
- Mice were infected with P. multocida.
- High-throughput microarray transcriptome analysis was performed on spleen, liver, and lung tissues.
- Differentially expressed genes (DEGs) were identified at early and late infection stages.
Main Results:
- Numerous DEGs were identified in infected organs.
- Enriched pathways included Toll-like receptor (TLR), NF-κB, MAPK, TNF, JAK-STAT, and NOD-like receptor signaling.
- Protein-protein interaction analysis suggested TLR4/CD14 recognition initiates signaling cascades via MyD88, IRAKs, and TRAF6, activating NF-κB and MAPK pathways.
Conclusions:
- P. multocida infection activates complex host immune signaling networks.
- TLR signaling plays a central role in recognizing P. multocida and initiating inflammatory responses.
- Identifying these pathways provides targets for potential therapeutic interventions.
