Carbon dioxide water-bath treatment augments peripheral blood flow through the development of angiogenesis

In this study, we investigated the effects of CO₂ water-bath therapy on blood flow and angiogenesis in the ischemic hind limb, as well as some plasma angiogenic factors in peripheral ischemic model. The hind limb ischemia was induced by occluding the femoral artery for 2 weeks in rats and treated with or without CO₂ water-bath therapy at 37 °C for 4 weeks (20 min treatment every day for 5 days per week). The peak blood flow and minimal and mean blood flow in the ischemic skeletal muscle were markedly increased by the CO₂ water-bath therapy. This increase in blood flow was associated with development of angiogenesis in the muscle, as well as reduction in the ischemia-induced increase in plasma malondialdehyde levels. Although plasma vascular endothelial growth factor and nitric oxide levels were increased in animals with peripheral ischemia, the changes in these biomarkers were not affected by CO₂ water-bath therapy. These results suggest that augmentation of blood flow in the ischemic hind limb by CO₂ water-bath therapy may be due to the development of angiogenesis and reduction in oxidative stress.