In Vitro Synthesis of Connectin in an Extract of Chicken Embryo Muscles

Incorporation of ³⁵S-methionine into connectin during protein synthesis in an extract from chicken embryo muscles was detected by autoradiography after immunoprecipitation with anti-connectin antibodies followed by SDS gel electrophoresis. Any radioactivity of peptides smaller than connectin but larger than actin was not detected with anti-connectin antibodies reacted samples. On the other hand, incorporation of radioactive methionine into nebulin was not observed.