Algevir: An Expression System for Microalgae Based on Viral Vectors.

Area of Science:

• Biotechnology
• Molecular Biology
• Algal Research

Background:

• Microalgae are valuable platforms for producing compounds, but efficient expression systems are lacking.
• Existing methods for recombinant protein production in microalgae face challenges like low yields and instability.
• Novel expression strategies are crucial for expanding microalgal biotechnology applications.

Purpose of the Study:

• To explore the use of viral expression vectors in microalgae for the first time.
• To develop an efficient and inducible system for high-level recombinant protein production.
• To overcome limitations of current nuclear transformation methods in microalgae.

Main Methods:

• Development of an inducible geminiviral vector (Algevir) for microalgae.
• Utilizing Rep-mediated replication for expression cassette amplification.
• Production and analysis of complex viral proteins and bacterial toxin subunits.

Main Results:

• The Algevir system achieved high-level production of antigenic proteins.
• Functional recombinant proteins, including Zaire ebolavirus GP1 and E. coli heat-labile enterotoxin B subunit, were produced.
• A maximum yield of 1.25 mg/g fresh biomass (6 mg/L) was obtained within 3 days post-transformation.

Conclusions:

• The Algevir system offers a fast and efficient method for recombinant protein production in microalgae.
• This viral vector approach surpasses challenges associated with low yields and unstable expression in nuclear transformants.
• Algevir facilitates the production of recombinant proteins, even those that are toxic or complex.