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Updated: Feb 26, 2026
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
miR‑140‑5p inhibits human glioma cell growth and invasion by targeting JAG1
Huan-Li Yang1, Ya-Mei Gao2, Jin-An Zhao3
1Disinfection Supply Room, Weinan Central Hospital, Weinan, Shaanxi 714000, P.R. China.
Abstract:
miR‑140‑5p has been reported to be a tumor suppressor in several types of human cancer, however, little is known about its expression and function in human gliomas. The present study aimed to detect the expression of miR‑140‑5p in human glioma tissues and cell lines, and to investigate the effect of miR‑140‑5p on glioma cell growth, invasion and adhesion using in vitro gain‑of‑function and loss‑of‑function experiments. Furthermore, the hypothesis that Jagged1 (JAG1) may be a target gene of miR‑140‑5p was tested. Reverse transcription‑quantitative polymerase chain reaction analysis revealed that miR‑140‑5p was significantly downregulated in human glioma tissues and cell lines compared with normal tissues, and that its expression was correlated with the grade of gliomas. Transfection of a miR‑140‑5p mimic into SW1783 glioma cells promoted cell growth, invasion and adhesion, as determined by MTT, Transwell and cell adhesion assays respectively. By contrast, transfection of a miR‑140‑5p inhibitor had the opposite effect. A dual‑luciferase reporter assay confirmed that JAG1 was a target gene of miR‑140‑5p, and miR‑140‑5p inhibited JAG1 expression both at the mRNA and protein level. In addition, JAG1 overexpression reversed the effect of miR‑140‑5p on glioma cell growth, invasion and adhesion. In conclusion, the present study is the first to reveal that miR‑140‑5p acts as a tumor suppressor in human gliomas. JAG1 was demonstrated to be a novel target of miR‑140‑5p, and miR‑140‑5p exerted its inhibitory effect on human glioma growth and invasion, partly by suppressing JAG1. The present study may provide useful information toward novel targets for the treatment of gliomas.
Insights
MicroRNA-140-5p (miR-140-5p) functions as a tumor suppressor in human gliomas, inhibiting cell growth and invasion. It targets Jagged1 (JAG1), suggesting potential therapeutic strategies for glioma treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNA-140-5p (miR-140-5p) is a known tumor suppressor in various cancers, but its role in human gliomas is largely unexplored.
- Gliomas are primary brain tumors with significant morbidity and mortality, necessitating research into novel therapeutic targets.
Purpose of the Study:
- To investigate the expression profile of miR-140-5p in human glioma tissues and cell lines.
- To elucidate the functional role of miR-140-5p in regulating glioma cell proliferation, invasion, and adhesion.
- To identify and validate Jagged1 (JAG1) as a direct target of miR-140-5p in glioma.
Main Methods:
- Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for miR-140-5p expression analysis.
- In vitro gain-of-function (mimic transfection) and loss-of-function (inhibitor transfection) experiments.
- Cell proliferation (MTT), invasion (Transwell), and adhesion assays.
- Dual-luciferase reporter assays to confirm JAG1 as a miR-140-5p target.
Main Results:
- miR-140-5p expression was significantly downregulated in glioma tissues and cell lines, correlating with higher tumor grade.
- Overexpression of miR-140-5p promoted glioma cell growth, invasion, and adhesion, while inhibition had the opposite effect.
- JAG1 was confirmed as a direct target of miR-140-5p, with miR-140-5p suppressing JAG1 expression at both mRNA and protein levels.
- JAG1 overexpression counteracted the inhibitory effects of miR-140-5p on glioma cell behavior.
Conclusions:
- miR-140-5p acts as a tumor suppressor in human gliomas.
- JAG1 is a novel target of miR-140-5p, mediating its tumor-suppressive functions.
- miR-140-5p inhibits glioma progression, partly through JAG1 suppression, offering potential therapeutic avenues.
