Related Experiment Video
Updated: Feb 26, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Association between interleukin-32 polymorphism and multiple sclerosis
Zaher Morsaljahan1, Alireza Rafiei2, Reza Valadan2
1Department of Immunology, Mazandaran University of Medical Sciences, Sari, Iran; Molecular and Cell Biology Research Center, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Elevated serum levels of Interleukin-32 (IL-32) and the presence of its C allele promoter polymorphism are associated with increased Multiple Sclerosis (MS) risk. This suggests IL-32 plays a role in MS pathogenesis.
Area of Science:
- Immunology
- Genetics
- Neurology
Background:
- Multiple Sclerosis (MS) is a chronic CNS inflammatory disease influenced by genetic and environmental factors.
- Interleukin-32 (IL-32) is a cytokine known to induce key inflammatory mediators.
- The role of IL-32 in MS pathogenesis requires further investigation.
Purpose of the Study:
- To investigate the association between IL-32 serum levels and its C/T promoter Single Nucleotide Polymorphism (SNP) with susceptibility to Multiple Sclerosis (MS).
Main Methods:
- A case-control study involving 132 MS patients and 172 healthy controls.
- Serum IL-32 levels were measured using ELISA.
- IL-32 gene promoter C/T polymorphism was analyzed using RFLP-PCR.
Main Results:
- MS patients exhibited significantly higher serum IL-32 levels compared to healthy controls (p=0.001).
- The C allele of the IL-32 promoter polymorphism was more prevalent in MS patients, potentially increasing MS risk by 1.6-fold (p=0.038).
- The presence of the C allele was linked to significantly enhanced IL-32 production.
Conclusions:
- This study is the first to examine IL-32 gene promoter C/T polymorphism and serum levels in MS.
- Increased IL-32 serum levels, potentially amplified by the C allele, suggest IL-32 is a key factor in MS immunodysregulation and pathogenesis.
Related Concept Videos
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase
Single Nucleotide Polymorphisms-SNPs

