Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vitro blood-brain barrier models for the study of brain shuttle peptide transport.

Advances in pharmacology (San Diego, Calif.)·2026
Same author

Response to comments on "First record of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) isolated from the Meia Ponte River and effluent in Brazil: an analysis of 1198 isolates".

New microbes and new infections·2026
Same author

Digitally Coded, Screen-Printed Flexible Metasurfaces for Tunable Electromagnetic Responses.

ACS applied materials & interfaces·2026
Same author

Advanced microfluidic and 3D cell culture platforms for modeling vascularization in diabetic foot ulcers: A systematic review of translational challenges and perspectives.

PloS one·2026
Same author

A comprehensive analysis of the SARS-CoV-2 omicron variant in Tocantins State, Brazil, and tracing the spread of the XBB.1.18.1 lineage.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]·2026
Same author

Nanoyeast-based impedimetric biosensor with mutated single chain antigen-binding fragment anchoring for SARS-CoV-2 detection.

Biomedical microdevices·2026

Related Experiment Video

Updated: Feb 26, 2026

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
09:05

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

5.6K

Loop-mediated isothermal amplification in disposable polyester-toner microdevices.

Kezia Gomes de Oliveira1, Juliane Cristina Borba2, Alexandre Melo Bailão3

  • 1Instituto de Química, Universidade Federal de Goiás, Goiânia, GO 74690-900, Brazil.

Analytical Biochemistry
|July 19, 2017
PubMed
Summary
This summary is machine-generated.

Isothermal DNA amplification using Loop-mediated isothermal amplification (LAMP) in a polyester-toner microdevice offers a simple, rapid method for E. coli detection. This point-of-care diagnostic tool enhances molecular diagnostics in resource-limited settings.

Keywords:
E. coliLAMPPeT microdevicePoint-of-caremalB gene

More Related Videos

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

5.3K
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

9.1K

Related Experiment Videos

Last Updated: Feb 26, 2026

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
09:05

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

5.6K
Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

5.3K
A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

9.1K

Area of Science:

  • Molecular Diagnostics
  • Biotechnology
  • Microfluidics

Background:

  • Isothermal DNA amplification methods offer an alternative to traditional PCR in molecular diagnostics.
  • These methods simplify operations by eliminating heating cycles, reducing equipment costs, and enabling rapid results.
  • They are particularly beneficial for laboratories with limited resources.

Purpose of the Study:

  • To develop a simple and fast method for detecting E. coli using isothermal amplification.
  • To utilize a disposable polyester-toner (PeT) microdevice for E. coli detection.
  • To assess the efficiency and sensitivity of Loop-mediated isothermal amplification (LAMP) in a microchip format.

Main Methods:

  • Loop-mediated isothermal amplification (LAMP) of the malB gene from E. coli was performed in a 5-μL microchamber.
  • The reaction was maintained at 66°C for 60 minutes using a thermoblock.
  • Surface passivation of PeT channels with BSA was employed to enhance LAMP efficiency.
  • Amplicon detection was conducted both on-chip (visual) and off-chip (gel electrophoresis).

Main Results:

  • Visual detection on-chip identified as low as 10 double-stranded DNA copies of E. coli.
  • Gel electrophoresis off-chip detected amplicons from reactions initiated with only one double-stranded DNA copy.
  • BSA surface passivation improved the efficiency of the LAMP reaction in the PeT microdevice.

Conclusions:

  • LAMP performed in a PeT microchip is a viable and sensitive method for E. coli detection.
  • This approach offers a valuable tool for point-of-care molecular diagnostics.
  • The method's simplicity and speed make it suitable for resource-limited laboratory settings.