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Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Author Spotlight: Advancements in Stem Cell Regenerative Therapy Through Photobiomodulation

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Biomaterials for 4D stem cell culture.

Amber M Hilderbrand¹, Elisa M Ovadia¹, Matthew S Rehmann¹

¹Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA.

Current Opinion in Solid State & Materials Science

|July 19, 2017

Summary

Researchers are developing dynamic hydrogels to mimic the body's complex stem cell environments. These advanced biomaterials allow for controlled changes over time, improving the study of stem cell behavior and differentiation.

Keywords:

3D cell culture biomaterials click chemistry hydrogels stem cells

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Area of Science:

Biomaterials Science
Stem Cell Biology
Tissue Engineering

Background:

Stem cells exist in dynamic, three-dimensional (3D) in vivo environments that influence their function.
Replicating these complex, time-varying microenvironments in traditional cell culture has been a significant challenge.
Hydrogel-based biomaterials offer a promising platform due to their water content, elasticity, and tunable properties.

Purpose of the Study:

To review techniques for engineering hydrogels that dynamically change properties over time.
To explore the application of these dynamic hydrogels in mimicking in vivo stem cell niches.
To discuss the use of these 4D (three dimensions plus time) systems for studying stem cell processes.

Main Methods:

Formation of hydrogel-based biomaterials.
Engineering time-dependent property changes in hydrogels via addition, cleavage, or non-covalent reactions.
Utilizing these dynamic hydrogels for stem cell culture.

Main Results:

Hydrogels can be engineered to exhibit controlled changes in matrix properties over time.
These dynamic hydrogels effectively mimic aspects of native stem cell microenvironments.
Applications in studying stem cell migration and differentiation are demonstrated.

Conclusions:

Dynamic hydrogel systems provide advanced tools for creating biomimetic cell culture environments.
These 4D culture systems are crucial for understanding and directing stem cell fate and function.
Further development holds potential for regenerative medicine and disease modeling.