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Injectable scaffold materials differ in their cell instructive effects on primary human myoblasts.

Eva Kildall Hejbøl1, Jeeva Sellathurai1, Prabha Damodaran Nair2

  • 1Institute of Clinical Research, SDU Muscle Research Cluster, University of Southern Denmark, Odense, Denmark.

Journal of Tissue Engineering
|July 19, 2017
PubMed
Summary

Injectable scaffolds guide human myogenic cells into different states. The choice of scaffold material, including fibrin and alginate, influences cell proliferation and differentiation, impacting tissue regeneration potential.

Keywords:
Myoblastscell instructiveinjectable scaffoldsregeneration

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Area of Science:

  • Biomaterials science
  • Regenerative medicine
  • Cell biology

Background:

  • Injectable scaffolds are crucial for minimally invasive cell delivery in tissue regeneration.
  • Understanding scaffold-cell interactions is key to optimizing regenerative therapies.
  • Skeletal muscle repair benefits from effective cell delivery systems.

Purpose of the Study:

  • To investigate the in vitro instructive effects of three injectable scaffolds (fibrin, alginate, poly(lactic-co-glycolic acid)-based microparticles) on primary human myoblasts.
  • To analyze how different scaffold materials influence myoblast morphology, proliferation, and myogenic progression.
  • To assess the viability and functional capacity of myoblasts released from these scaffolds.

Main Methods:

  • In vitro culture of primary human myoblasts with fibrin, alginate, and poly(lactic-co-glycolic acid)-based microparticle scaffolds.
  • Microscopy to observe myoblast morphology and myogenic program progression.
  • Assessment of cell proliferation, differentiation, and release from scaffolds.

Main Results:

  • Scaffold material significantly altered myoblast behavior: alginate induced quiescence, fibrin promoted differentiation into myotubes, and poly(lactic-co-glycolic acid) supported prolonged proliferation.
  • Myoblasts released from alginate and fibrin scaffolds retained their ability to proliferate and differentiate.
  • Different injectable scaffolds induce distinct cellular states in human myoblasts.

Conclusions:

  • The choice of injectable scaffold material dictates the behavior and state of human myogenic cells.
  • This differential cell guidance offers potential for tailoring cell therapies for skeletal muscle regeneration.
  • Further in vivo studies are warranted to evaluate the regenerative potential based on scaffold-induced cell states.