Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Motion-aware imaging flow cytometry framework for robust counting and tracking of cells with non-uniform velocities in microfluidics.

Applied optics·2026
Same author

From the Clinic, to the Clinic: Improving the Fluorescent Imaging Quality of ICG via Amphiphilic NIR-IIa AIE Probe.

Biosensors·2026
Same author

EdgePhase: a portable edge-computational microscope for point-of-care quantitative phase imaging.

Optics express·2025
Same author

A Camera-Like Dual-Defocus Curvature Wavefront Sensor With GPU Acceleration for Real-Time Quantitative Phase Imaging.

Microscopy research and technique·2025
Same author

Dual-View Transport of Intensity Phase Imaging-Based Flow Cytometry for Label-Free Cell Analysis and Classification.

Journal of biophotonics·2025
Same author

Mitigating reconstruction errors in extended-ptychography with adaptive scanning position correction.

Applied optics·2025
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Feb 26, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

8.9K

Ultra-high speed digital micro-mirror device based ptychographic iterative engine method.

Aihui Sun1, Xiaoliang He2, Yan Kong1

  • 1Department of Optoelectronic Information Science and Engineering, School of Science, Jiangnan University, Wuxi, Jiangsu 214122, China.

Biomedical Optics Express
|July 19, 2017
PubMed
Summary
This summary is machine-generated.

Digital micro-mirror devices (DMD) significantly accelerate Ptychographic Iterative Engine (PIE) imaging by replacing mechanical scanning. This innovation drastically cuts data acquisition and reconstruction times while maintaining high resolution.

Keywords:
(100.5070) Phase retrieval(110.1650) Coherence imaging(110.1758) Computational imaging

More Related Videos

High-speed Particle Image Velocimetry Near Surfaces
11:59

High-speed Particle Image Velocimetry Near Surfaces

Published on: June 24, 2013

33.9K
Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.8K

Related Experiment Videos

Last Updated: Feb 26, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

8.9K
High-speed Particle Image Velocimetry Near Surfaces
11:59

High-speed Particle Image Velocimetry Near Surfaces

Published on: June 24, 2013

33.9K
Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

10.8K

Area of Science:

  • X-ray imaging
  • Computational microscopy
  • Materials science

Background:

  • Ptychographic Iterative Engine (PIE) offers high-resolution imaging but suffers from long data acquisition times due to mechanical scanning.
  • Mechanical scanning in PIE requires precise positioning, necessitating time-consuming error correction procedures.
  • The stability requirements of traditional PIE limit its practical applications.

Purpose of the Study:

  • To develop a faster and more robust PIE technique by replacing mechanical scanning with a digital micro-mirror device (DMD).
  • To reduce data acquisition and reconstruction times in PIE without compromising spatial resolution or field of view.
  • To enhance the applicability of PIE for various imaging tasks.

Main Methods:

  • Implemented a digital micro-mirror device (DMD) to generate fast scanning illumination patterns on the sample.
  • Replaced the conventional transverse mechanical scanning stage with the on/off switching capability of DMD micro-mirrors.
  • Utilized optical lithography for DMD fabrication, achieving precision higher than 10 nm.

Main Results:

  • Reduced data acquisition time from over 15 minutes to under 20 seconds for 120 diffraction patterns covering 147.08 mm².
  • Eliminated the need for position-error correction due to the high precision of DMD.
  • Achieved a spatial resolution of 5.52 μm with a field of view of 147.08 mm².
  • Demonstrated experimental validation using a USAF resolution target and a biological sample.

Conclusions:

  • DMD-based PIE overcomes the limitations of mechanical scanning in traditional PIE.
  • The method significantly accelerates data acquisition and reconstruction, improving system stability and practical applicability.
  • High resolution and large field of view imaging are achievable with the enhanced PIE technique.