Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Xenophagocytosis blockade enhances interspecies chimerism.

Cell·2026

Same author

Xenophagocytosis blockade enhances interspecies chimerism.

bioRxiv : the preprint server for biology·2025

Same author

Derivation of resident macrophages and construction of tumor microenvironment in Flk-1-knockout chimeric mice produced via blastocyst complementation.

Scientific reports·2025

Same author

Allogeneic, Xenogeneic, and Exogenic Hearts for Transplantation.

Methodist DeBakey cardiovascular journal·2025

Same author

Inhibition of PRC2 enables self-renewal of blastoid-competent naive pluripotent stem cells from chimpanzee.

Cell stem cell·2025

Same author

Intercellular mRNA transfer alters the human pluripotent stem cell state.

Proceedings of the National Academy of Sciences of the United States of America·2025

Search research articles

Related Experiment Video

Updated: Feb 26, 2026

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Published on: May 29, 2018

Interspecies chimeras for human stem cell research.

Hideki Masaki¹, Hiromitsu Nakauchi^2,3

¹Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.

Development (Cambridge, England)

|July 20, 2017

Summary

Interspecies chimeric assays help study human stem cells in other species. Refining these assays can advance understanding of cell potential, xenogeneic barriers, and human organ development.

Keywords:

Chimeric assays Heterochronicity Human development Interspecies chimera Pluripotent stem cell

More Related Videos

Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras

Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras

Published on: December 22, 2010

Author Spotlight: Exploring the Lifespan Dynamics of Healthy Human Hematopoiesis

Author Spotlight: Exploring the Lifespan Dynamics of Healthy Human Hematopoiesis

Published on: December 8, 2023

Related Experiment Videos

Last Updated: Feb 26, 2026

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Published on: May 29, 2018

Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras

Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras

Published on: December 22, 2010

Author Spotlight: Exploring the Lifespan Dynamics of Healthy Human Hematopoiesis

Author Spotlight: Exploring the Lifespan Dynamics of Healthy Human Hematopoiesis

Published on: December 8, 2023

Area of Science:

Stem cell biology
Developmental biology
Xenotransplantation research

Background:

Interspecies chimeric assays are crucial for evaluating human stem and progenitor cell potential.
Understanding cellular behavior across species is key to advancing regenerative medicine.

Purpose of the Study:

To discuss factors influencing interspecies chimera generation.
To propose strategies for improving chimera generation.
To highlight the potential applications of refined interspecies chimera models.

Main Methods:

Review of factors affecting chimera formation (evolutionary distance, developmental timing, apoptosis).
Discussion of potential strategies to overcome these barriers.

Main Results:

Identified key challenges in generating viable interspecies chimeras.
Proposed approaches to enhance the success rate of these assays.

Conclusions:

Optimizing interspecies chimera generation offers insights into cellular potential and xenogeneic interactions.
Refined methods can aid in modeling human development and creating transplantable organs.