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Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

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Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
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Single-component supported lipid bilayers probed using broadband nonlinear optics.

Laura L Olenick1, Hilary M Chase, Li Fu

  • 1Department of Chemistry, Northwestern University, Evanston, IL 60208, USA. geigerf@chem.northwestern.edu.

Physical Chemistry Chemical Physics : PCCP
|July 20, 2017
PubMed
Summary
This summary is machine-generated.

Broadband SFG spectroscopy enhances lipid bilayer analysis. This technique reveals alkyl chain tilt angles, offering improved signal-to-noise ratios for studying membrane structure and dynamics.

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Area of Science:

  • Biophysics
  • Spectroscopy
  • Materials Science

Background:

  • Supported lipid bilayers are crucial models for cell membranes.
  • Scanning SFG spectroscopy has limitations in signal-to-noise ratio.
  • Zwitterionic lipids with PC headgroups form well-defined bilayers.

Purpose of the Study:

  • To evaluate broadband SFG spectroscopy for probing lipid bilayers.
  • To characterize the structural properties of lipid bilayers using SFG.
  • To investigate spectral interferences in SFG measurements.

Main Methods:

  • Broadband SFG spectroscopy.
  • Synthesis and characterization of lipid bilayers (DOPC, POPC, DLPC, DMPC, DPPC, DSPC).
  • H2O-D2O exchange experiments.

Main Results:

  • Broadband SFG offers superior signal-to-noise ratios compared to scanning systems.
  • SFG spectra show characteristic peaks related to lipid chain structure and phase transitions.
  • Spectral interference from aqueous OH stretches affects lineshapes, even in broadband setups.
  • Alkyl chain terminal methyl groups are tilted 15°-35° from the surface normal in the absence of solvent interference.

Conclusions:

  • Broadband SFG spectroscopy is a powerful tool for lipid bilayer research.
  • Understanding spectral interference is key for accurate structural determination.
  • The study provides insights into the molecular orientation within lipid bilayers.