Specific fluorescence labeling of target proteins by using a ligand-4-azidophthalimide conjugate
Kosuke Chiba1, Miwako Asanuma, Minoru Ishikawa
1Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. yamaguchi@iam.u-tokyo.ac.jp.
Chemical Communications (Cambridge, England)
|July 21, 2017
Abstract:
We herein propose a simple affinity-labeling method using a ligand-4-azidophthalimide (AzPI) conjugate. As a proof of concept, we show that two different ligand-AzPI conjugates enabled highly specific fluorescence labeling of their individual target proteins even in crude cell lysates. This method was also applied to label endogenous target proteins inside living cells.
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