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Updated: Feb 26, 2026

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
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MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

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miR-483-5p promotes prostate cancer cell proliferation and invasion by targeting RBM5

Zhi-Gang Yang1, Xu-Dong Ma1, Zhao-Hui He2

  • 1Department of Urology, Baotou Central Hospital, Inner Mongolia Medical University, China.

International Braz J Urol : Official Journal of the Brazilian Society of Urology
|July 21, 2017
PubMed
Abstract

Insights

MicroRNA-483-5p (miR-483-5p) is upregulated in prostate cancer, promoting cell growth and invasion. It targets RBM5, suggesting a miR-483-5p/RBM5 pathway in prostate cancer development.

Area of Science:

  • Molecular Oncology
  • Cancer Biology
  • Gene Regulation

Background:

  • MicroRNA-483-5p (miR-483-5p) is implicated as a miRNA oncogene in various cancers.
  • The specific role of miR-483-5p in prostate cancer pathogenesis remains underexplored.

Purpose of the Study:

  • To investigate the function of miR-483-5p in prostate cancer.
  • To elucidate the regulatory relationship between miR-483-5p and RBM5 in prostate cancer cells.

Main Methods:

  • Quantitative real-time PCR for miR-483-5p expression analysis.
  • MTT and trans-well invasion assays to assess proliferation and invasion.
  • Western blotting for protein expression analysis.
  • Luciferase reporter assays to confirm RBM5 as a direct target of miR-483-5p.

Main Results:

  • miR-483-5p was significantly upregulated in prostate cancer cell lines and tissues.
  • Inhibition of miR-483-5p suppressed prostate cancer cell proliferation and invasion.
  • miR-483-5p directly targets the 3' untranslated region of RBM5.
  • RBM5 overexpression counteracted the pro-growth and pro-invasion effects of miR-483-5p.

Conclusions:

  • A novel miR-483-5p/RBM5 regulatory axis is identified in prostate cancer.
  • This pathway contributes to the development and progression of prostate cancer.
Keywords:
MIRN483 microRNA, human [Supplementary Concept]Prostatic NeoplasmsRBM5 protein, human [Supplementary Concept]

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