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Bridged Rebar Graphene functionalized aptasensor for pathogenic E. coli O78:K80:H11 detection
Harmanjit Kaur1, Munish Shorie1, Manju Sharma1
1Institute of Nano Science & Technology, Mohali 160062, India.
Biosensors & Bioelectronics
|July 21, 2017
Summary
We developed a new aptasensor using Bridged Rebar Graphene (BRG) for detecting pathogenic bacteria E. coli. This label-free sensor achieves high sensitivity for E. coli O78:K80:H11 in various food and water samples.
Area of Science:
- Nanotechnology
- Biosensing
- Materials Science
Background:
- Pathogenic bacteria detection is crucial for public health.
- Existing methods often require labels or are less sensitive.
- Need for rapid, sensitive, and label-free detection strategies.
Purpose of the Study:
- To develop a novel nanostructured aptasensor for label-free impedimetric sensing of E. coli O78:K80:H11.
- To fabricate functionalized Bridged Rebar Graphene (BRG) for enhanced sensor performance.
Main Methods:
- Fabrication of 3D-hierarchical BRG nanoconstructs via MWCNT unscrolling and TPA bridging.
- Screening of specific anti-E. coli DNA aptamers using in-situ SELEX.
- Impedimetric detection of bacteria-DNA interactions on the BRG aptasensor.
Main Results:
- The BRG nanoconstruct showed synergistic effects with enhanced electrical properties and stable bio-interface.
- The aptasensor achieved a low detection limit of ~10^1 cfu/mL for E. coli O78:K80:H11.
- A dynamic response range of 10^1 to 10^6 cfu/mL was observed in water, juice, and milk samples.
Conclusions:
- The developed nanostructured aptasensor offers a sensitive and label-free platform for E. coli detection.
- BRG nanoconstructs are promising for advanced biosensing applications.
- This method provides a viable tool for monitoring bacterial contamination in food and beverages.
