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miR‑4262 inhibits colon cancer cell proliferation via targeting of GALNT4
Jian-Jun Qu1, Xiang-Yang Qu2, De-Zhen Zhou1
1Department of Surgical Oncology, The People's Hospital of Weifang, Weifang, Shandong 261000, P.R.China.
Abstract:
MicroRNAs (miRs) have been demonstrated to be important in the establishment and progression of colon cancer. However the underlying molecular mechanisms remain to be fully elucidated. Polypeptide N‑acetylgalactosaminyltransferase4 (GALNT4) participates in numerous cellular processes, including tumorigenesis. The present study used reverse transcription‑quantitative polymerase chain reaction and western blotting to investigate the expression levels of miR‑4262 and GALNT4 in tissues and cells. In addition, MTS and colony formation assays, and cell cycle analysis were performed to evaluate the effect of miR‑4262 on cell proliferation and the cell cycle. The findings demonstrated that miR‑4262 was a direct target of GALNT4 mRNA. Overexpression of miR‑4262 was demonstrated to decrease GALNT4 mRNA and protein expression levels, and thereby suppressed cell viability, growth and cell‑cycle progression in SW480 and SW620 colon cancer cells. In addition, knockdown of miR‑4262 significantly increased the cell viability, growth, and cell‑cycle progression of SW480 and SW620 cells. The expression level of miR‑4262 was observed to be downregulated as the expression of GALNT4 was upregulated in colon cancer tissues and cell lines. In conclusion, the results demonstrated that miR‑4262 may be involved in the development of colon cancer via targeting of GALNT4. The miR‑4262/GALNT4 axis may be a novel target for diagnosing and understanding the underlying molecular mechanism of colon cancer.
Insights
MicroRNA-4262 (miR-4262) suppresses colon cancer growth by targeting Polypeptide N‑acetylgalactosaminyltransferase4 (GALNT4). This miR-4262/GALNT4 interaction offers a potential new target for colon cancer diagnosis and treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRs) play a crucial role in colon cancer development and progression.
- The specific molecular mechanisms underlying miR involvement in colon cancer require further investigation.
- Polypeptide N‑acetylgalactosaminyltransferase4 (GALNT4) is implicated in various cellular processes, including tumorigenesis.
Purpose of the Study:
- To investigate the role of miR-4262 and GALNT4 in colon cancer.
- To elucidate the molecular mechanism by which miR-4262 affects colon cancer cell proliferation and cell cycle.
- To determine the relationship between miR-4262 and GALNT4 expression in colon cancer.
Main Methods:
- Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting were used to assess miR-4262 and GALNT4 expression.
- MTS and colony formation assays were performed to evaluate cell viability and growth.
- Cell cycle analysis was conducted to determine the impact on cell cycle progression.
Main Results:
- miR-4262 directly targets GALNT4 mRNA.
- Overexpression of miR-4262 reduced GALNT4 expression and suppressed colon cancer cell viability, growth, and cell cycle progression.
- Knockdown of miR-4262 increased colon cancer cell viability, growth, and cell cycle progression.
- miR-4262 was downregulated while GALNT4 was upregulated in colon cancer tissues and cell lines.
Conclusions:
- miR-4262 plays a significant role in colon cancer development by targeting GALNT4.
- The miR-4262/GALNT4 axis represents a potential novel target for colon cancer diagnosis and understanding its molecular mechanisms.
