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Colloidal precipitates

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

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Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

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Enzyme adsorption-induced activity changes: a quantitative study on TiO₂ model agglomerates.

Augusto Márquez¹, Krisztina Kocsis¹, Gregor Zickler¹

¹Department of Chemistry and Physics of Materials, Paris Lodron University of Salzburg, Jakob-Haringer-Strasse 2a, 5020, Salzburg, Austria.

Journal of Nanobiotechnology

|July 23, 2017

Summary

This summary is machine-generated.

Enzyme adsorption on titanium dioxide nanoparticles significantly reduces activity due to structural changes. Well-defined model systems are crucial for studying enzyme-nanoparticle interactions and activity retention.

Keywords:

Adsorption Agglomerates Enzymatic activity IR spectroscopy Nanoparticles TiO2 β-galactosidase

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Area of Science:

Biomaterials Science
Nanotechnology
Enzyme Kinetics

Background:

Enzyme activity on nanostructures is influenced by support properties, medium composition, and enzyme loading.
Understanding these interactions requires well-defined model systems for accurate characterization.

Purpose of the Study:

To investigate the activity retention of beta-galactosidase (beta-gal) adsorbed onto anatase titanium dioxide (TiO2) nanoparticles.
To elucidate the structural changes in beta-gal upon adsorption and their impact on enzymatic function.

Main Methods:

Preparation of aqueous dispersions of vapor phase-grown, thermally processed anatase TiO2 nanoparticles.
Characterization using dynamic light scattering and laser Doppler electrophoresis.
Enzyme adsorption studies and activity assays.
Attenuated Total Reflection Infrared (ATR-IR) spectroscopy to analyze protein secondary structure.

Main Results:

Complete adsorption of beta-gal onto TiO2 nanoparticles, forming heteroaggregates.
Significant loss of enzymatic activity (60-100 fold) at low enzyme loadings.
ATR-IR spectroscopy revealed a decrease in beta-sheet content and increased random structures in adsorbed beta-gal, indicating denaturation.

Conclusions:

Adsorption onto TiO2 nanoparticles induces structural changes in beta-gal, leading to substantial activity loss.
Well-defined model systems like anatase TiO2 nanoparticle agglomerates are essential for reliable studies on enzyme adsorption and activity.
Findings highlight the importance of considering enzyme structural integrity and support characteristics in nanobiotechnology applications.