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Multiplexed capillary microfluidic immunoassay with smartphone data acquisition for parallel mycotoxin detection.

Jessica M D Machado1, Ruben R G Soares2, Virginia Chu1

  • 1Instituto de Engenharia de Sistemas e Computadores - Microsistemas e Nanotecnologias (INESC MN) and IN - Institute of Nanoscience and Nanotechnology, Lisbon, Portugal.

Biosensors & Bioelectronics
|July 24, 2017
PubMed
Summary
This summary is machine-generated.

This study presents a microfluidic lab-on-a-chip device for rapid, multiplexed detection of harmful food contaminants ochratoxin A (OTA), aflatoxin B1 (AFB1), and deoxynivalenol (DON). The user-friendly system uses smartphone imaging for semi-quantitative analysis, meeting regulatory limits.

Keywords:
CapillarityColorimetryMicrofluidicsMultiplexingMycotoxinsSmartphone

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Area of Science:

  • Microfluidics
  • Lab-on-a-chip technology
  • Biosensing

Background:

  • Microfluidics enables portable lab-on-a-chip systems.
  • Capillarity simplifies fluidic manipulation for user-friendly devices.
  • Point-of-need analyses require sensitive and rapid detection methods.

Purpose of the Study:

  • To develop a microfluidic chip for simultaneous detection of mycotoxins.
  • To utilize smartphone imaging for data acquisition and analysis.
  • To assess the device's performance and validation in real samples.

Main Methods:

  • A polydimethylsiloxane (PDMS) microchannel-based autonomous capillary chip was fabricated.
  • A multiplexed, semi-quantitative colorimetric immunoassay was performed.
  • Smartphone camera and grayscale quantification were used for results analysis.

Main Results:

  • Simultaneous detection of ochratoxin A (OTA), aflatoxin B1 (AFB1), and deoxynivalenol (DON) was achieved within 10 minutes.
  • Sensitivities of <40 ng/mL (OTA), 0.1-0.2 ng/mL (AFB1), and <10 ng/mL (DON) were obtained.
  • The assay was successfully validated for mycotoxin detection in a corn-based feed sample.

Conclusions:

  • The developed microfluidic chip offers a user-friendly, portable solution for multiplexed mycotoxin detection.
  • The system meets regulatory limits for OTA, AFB1, and DON.
  • The device shows potential for real-world food safety applications.