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Liver Regeneration

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Updated: Feb 26, 2026

Isolation of Neonatal Extrahepatic Cholangiocytes

Isolation of Neonatal Extrahepatic Cholangiocytes

Published on: June 5, 2014

Cholangiocytes: Cell transplantation.

Lorenzo Ridola¹, Maria Consiglia Bragazzi¹, Vincenzo Cardinale¹

¹Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino, Sapienza University of Rome, Italy.

Biochimica Et Biophysica Acta. Molecular Basis of Disease

|July 24, 2017

Summary

Human biliary tree stem cells (hBTSCs) show promise as an ideal cell source for liver disease therapy, offering significant benefits without adverse events in initial cirrhotic patient trials.

Keywords:

Biliary tree stem cells Grafting Hepatic stem cells Hepatocytes Liver cell therapy Mesenchymal stem cells

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Last Updated: Feb 26, 2026

Isolation of Neonatal Extrahepatic Cholangiocytes

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Published on: June 5, 2014

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Published on: August 29, 2016

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Published on: February 10, 2018

Area of Science:

Hepatology
Stem Cell Biology
Regenerative Medicine

Background:

Orthotropic liver transplantation faces access limitations, driving interest in cell therapies for liver diseases.
Existing cell therapies have limitations, including the need for immunosuppression with adult hepatocytes and limited efficacy of mesenchymal and hematopoietic stem cells.

Purpose of the Study:

To review current literature on cell therapy for liver diseases.
To evaluate the advantages and disadvantages of various cell sources used in clinical trials.
To explore the potential benefits of human biliary tree stem cells (hBTSCs) for liver disease treatment.

Main Methods:

Literature review of cell therapy for liver diseases.
Analysis of clinical trial data for different stem cell sources.
Identification and characterization of multipotent stem cells in human biliary and gallbladder tissues.

Main Results:

Adult hepatocyte transplantation provides transient benefits but requires immunosuppression.
Mesenchymal and hematopoietic stem cells modulate autoimmune/inflammatory processes rather than replacing hepatocytes.
Multipotent stem cells expressing endodermal markers were identified in human biliary tree and gallbladder epithelium.
Initial clinical trials using hBTSCs in cirrhotic patients showed no adverse events and significant benefits.

Conclusions:

Human biliary tree stem cells (hBTSCs) represent a promising and potentially ideal cell source for liver disease therapy.
hBTSCs demonstrated safety and efficacy in early-stage cirrhotic patient treatment.
Further research into hBTSCs could offer new therapeutic avenues for advanced liver diseases.