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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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Immunophenotyping by Multiparameter Flow Cytometry.

Weina Chen1, Hung S Luu2,3

  • 1Department of Pathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, USA. Weina.Chen@utsouthwestern.edu.

Methods in Molecular Biology (Clifton, N.J.)
|July 24, 2017
PubMed
Summary
This summary is machine-generated.

Multiparameter flow cytometry is crucial for diagnosing acute myeloid leukemia (AML). This technique identifies unique immunophenotypic abnormalities in leukemic cells for classification and prognosis.

Keywords:
Acute myeloid leukemiaCytogeneticsFlow cytometryImmunophenotypingResidual disease

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Area of Science:

  • Hematology
  • Immunology
  • Oncology

Background:

  • Multiparameter flow cytometry is essential for acute myeloid leukemia (AML) diagnosis.
  • It detects immunophenotypic abnormalities in leukemic cell subpopulations.

Purpose of the Study:

  • To outline procedures for AML immunophenotyping.
  • To describe data analysis strategies for AML diagnosis and monitoring.

Main Methods:

  • Specimen processing and staining protocols.
  • Immunophenotyping using multiparameter flow cytometry.
  • Data analysis strategies for AML classification.

Main Results:

  • Immunophenotypic profiles serve as a "fingerprint" for AML.
  • Abnormalities help determine blast immaturity, lineage, and genetic factors.
  • Established profiles are vital for assessing residual disease.

Conclusions:

  • Flow cytometry is indispensable for AML diagnosis and classification.
  • Immunophenotyping aids in predicting prognosis and monitoring treatment response.