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Updated: Feb 26, 2026

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter
Published on: June 13, 2025
Automated clean-up, separation and detection of polycyclic aromatic hydrocarbons in particulate matter extracts using
Hwanmi Lim1, Trifa M Ahmed1,2, Christoffer Bergvall1,3
1Department of Environmental Science and Analytical Chemistry (ACES), Stockholm University, 106 91, Stockholm, Sweden.
Abstract:
An online two-dimensional (2D) liquid chromatography/2D gas chromatography system with two mass-selective detectors has been developed on the basis of a previous system with two flame ionization detectors. The method translation involved the change of carrier gas from hydrogen to helium, column dimension and detectors. The 2D system with two mass-selective detectors was validated with use of polycyclic aromatic hydrocarbon (PAH) standards and two standard reference materials from air and diesel exhaust. Furthermore, the system was applied to a real sample, wood smoke particulates. The PAH values determined correlated well with the previous data and those from the National Institute of Standards and Technology. The system enhanced the benefits of the previous system, which were limited by the low detectability and lack of mass selectivity. This study shows an automated 2D system that is valid for PAH analysis of complex environmental samples directly from crude extracts. Graphical Abstract Schematic illustration showing on-line clean-up, separation and detection using 2D-LC/2D-GC/MS.
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