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Integrative analysis of mRNA and miRNA expression profiles in oral lichen planus: preliminary results
Junjun Chen1, Guanhuan Du2, Yufeng Wang2
1Department of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory of Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Objective:
Oral lichen planus (OLP), a chronic inflammatory disease of unknown etiology, is considered a potentially malignant oral disorder. The aim of the present study was to analyze candidate microRNAs (miRNAs) and genes from patients with OLP and healthy controls (HCs).
Study Design:
Biopsy specimens of the oral mucosa were collected from patients with OLP (n = 9) and from HCs (n = 4). Differentially expressed miRNAs and differentially expressed genes were screened by using next-generation sequencing with DESeq and edgeR software algorithms.
Results:
A total of 94 differentially expressed miRNAs and 599 differentially expressed genes were detected in OLP. Potential regulatory miRNAs and genes were obtained by analyzing miRNA-messenger RNA networks. Of these, 5 downregulated miRNAs-Hsa-miR-135 a-5 p (P = .33), hsa-miR-128-3 p (P = .03), hsa-miR-218-5 p (P = .01), hsa-miR-125 a-5 p (P = .01), and hsa-let-7 e-5 p (P = .04)-were the most promising biomarkers in patients with OLP compared with HCs. The identified differentially expressed genes were significantly enriched in "inflammatory" events and immune-related terms through Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analysis.
Conclusions:
The integrative analysis of messenger RNA and miRNA profiles provides important information to elucidate gene expression mechanisms and a comprehensive perspective to study the etiology and pathogenesis of OLP.
