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Preparation of Samples for Electron Microscopy

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Author Spotlight: Advancing Tear Fluid Analysis Using a Standardized Protocol for Proteomics Research

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Published on: December 1, 2023

A Dry Method for Preserving Tear Protein Samples.

Weiwei Qin¹, Chan Zhao², Linpei Zhang¹

¹1 Department of Biochemistry and Molecular Biology, Beijing Normal University , Beijing, China .

Biopreservation and Biobanking

|July 27, 2017

Summary

A new dry method preserves tear proteins for biomarker research. This simple technique allows room-temperature storage and large-scale sample availability for disease detection.

Keywords:

biobanking proteomics tear

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Published on: February 27, 2020

Area of Science:

Biochemistry
Biomarker Research
Ophthalmology

Background:

Tears are crucial biofluids containing numerous molecules for disease biomarker research.
Current tear sample storage methods can be complex and costly.
Efficient, low-cost tear sample preservation is needed for large-scale biomarker studies.

Purpose of the Study:

To develop and validate a novel, simple, low-cost, and efficient dry method for storing tear samples.
To assess the preservation of tear protein patterns using the developed dry method.
To evaluate the feasibility of using tears as a window for monitoring tissue and organ health.

Main Methods:

Development of a Schirmer's strip-based dry method for tear sample collection and storage in vacuum bags at room temperature.
Liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis to compare proteins recovered from dry-stored samples versus traditional wet-stored samples.
Identification of tissue/organ-enriched proteins in tear samples.

Main Results:

The dry method successfully preserved tear protein patterns.
No significant differences were observed in protein analysis between the dry and wet storage methods.
Several tissue/organ-enriched proteins were identified in tear samples, suggesting tears can reflect systemic changes.

Conclusions:

The novel dry method offers a simple, cost-effective, and efficient approach for tear sample preservation.
This method facilitates large-scale sample storage and transportation, increasing accessibility for tear biomarker research.
Tears hold potential as a non-invasive window for monitoring various tissue and organ health conditions through biomarker analysis.