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Related Experiment Video

Updated: Feb 25, 2026

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Published on: September 28, 2018

High throughput automated microbial bioreactor system used for clone selection and rapid scale-down process

M Lourdes Velez-Suberbie¹, John P J Betts², Kelly L Walker³

¹The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Gower Street, Bernard Katz Building, London, WC1E 6BT, U.K.

Biotechnology Progress

|July 28, 2017

Summary

This summary is machine-generated.

High throughput microbioreactor systems like the ambr 15f enable efficient microbial fermentation for bioprocess development. This automated system supports strain and molecule selection, demonstrating robust cell growth and production comparable to larger bioreactors.

Keywords:

E. coli ambr 15 fermentation high throughput scale-down single use microbioreactor

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Area of Science:

Biotechnology
Biochemical Engineering
Microbial Fermentation

Background:

High throughput automated fermentation systems are crucial for early bioprocess development.
Microbioreactors offer a scalable solution for microbial culture optimization.

Purpose of the Study:

To evaluate the ambr 15f, a 24 x 15 mL single-use microbioreactor system, for microbial fed-batch fermentation.
To compare its performance for Escherichia coli strains and the production of hGH and scFv.
To assess scalability and cell robustness compared to traditional bioreactors.

Main Methods:

Utilized the ambr 15f system for fed-batch cultures of E. coli BL21 (DE3) and MC4100.
Tested various carbon sources with automated bolus, linear, and exponential feeding strategies.
Employed power per unit volume (P/V) for scale-up comparison and shear stress tests.

Main Results:

Demonstrated the ambr 15f system's capacity for automated feeding and handling diverse carbon sources.
Achieved comparable growth and product yield to larger bioreactors, suggesting a 1,300-fold scale comparability.
Confirmed cell robustness under shear stress conditions similar to industrial centrifuges.

Conclusions:

The ambr 15f is an efficient high throughput microbial system for bioprocess development.
It facilitates rapid strain and molecule selection and enables effective scale-up strategies.
Results support the utility of microbioreactors for mimicking industrial fermentation conditions.