Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

4.0K
Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
4.0K
Cognitive Development During Adulthood01:30

Cognitive Development During Adulthood

973
Cognitive development continues throughout adulthood, undergoing significant shifts across early, middle, and late stages. Individual transition occurs from adolescent idealism to pragmatic and adaptable thinking in early adulthood. During this period, individuals learn to integrate personal beliefs with the recognition that other perspectives are equally valid. Exposure to the complexities of modern society, diverse experiences, and higher education contribute to this adaptive thought process,...
973
Protein Networks02:26

Protein Networks

2.9K
2.9K
Protein Networks02:26

Protein Networks

4.6K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.6K
Aging01:26

Aging

894
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
894

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HTZ-1/H2A.Z expression sustains transcriptional programs that regulate Caenorhabditis elegans lifespan.

Mechanisms of ageing and development·2026
Same author

AI-guided analysis of human pancreatic islet sociology reveals distinct cell compositional changes in type 1 diabetes.

bioRxiv : the preprint server for biology·2026
Same author

Adaptive Fisher's method using weakly geometric grid for combining <i>p</i>-values with application to COVID-19 surveillance.

Journal of the Royal Statistical Society. Series C, Applied statistics·2026
Same author

Molecular Characterization of the Progressive Landscape of Depression.

bioRxiv : the preprint server for biology·2026
Same author

Re-thinking neuropeptide therapeutics: What Neuropeptide Y and Galanin teach us about stress, resilience, and drug design.

Neuropeptides·2026
Same author

The α5-Containing GABA<sub>A</sub> Receptor as a Target for Disorders of Altered Dendritic Inhibition.

Biological psychiatry·2026

Related Experiment Video

Updated: Feb 25, 2026

Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer&#39;s Disease
04:22

Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer's Disease

Published on: May 20, 2024

1.5K

Resilient protein co-expression network in male orbitofrontal cortex layer 2/3 during human aging.

Mohan Pabba1, Enzo Scifo1, Fenika Kapadia1

  • 1Campbell Family Mental Health Research Institute of CAMH, Neurobiology of Depression and Aging, Toronto, Ontario, Canada.

Neurobiology of Aging
|July 28, 2017
PubMed
Summary

This study reveals key protein changes in the aging orbitofrontal cortex (OFC), highlighting altered cell communication and nutrient sensing. Despite these changes, brain

Keywords:
CortexLayer 2/3Normal agingProteomics and RNA-Seq

More Related Videos

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

3.3K
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

9.0K

Related Experiment Videos

Last Updated: Feb 25, 2026

Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer&#39;s Disease
04:22

Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer's Disease

Published on: May 20, 2024

1.5K
Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

3.3K
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

9.0K

Area of Science:

  • Neuroscience
  • Proteomics
  • Aging Research

Background:

  • The orbitofrontal cortex (OFC) is susceptible to aging processes, both normal and pathological.
  • Large-scale proteomic studies with layer resolution are lacking for age-related changes in the OFC.

Purpose of the Study:

  • To perform a large-scale proteomic analysis of human OFC layer 2/3 to identify age-related protein alterations.
  • To investigate the functional implications of identified differentially expressed proteins in the context of aging hallmarks and neurological disorders.

Main Methods:

  • High-throughput mass spectrometry-based proteomic analysis was conducted on OFC layer 2/3 samples.
  • Samples were obtained from two age groups: 'young' (15-43 years) and 'old' (62-88 years) human males.
  • Statistical analysis identified differentially expressed proteins (DEPs) based on p-value and effect size.

Main Results:

  • A total of 4193 proteins were detected, with 127 identified as differentially expressed between young and old subjects.
  • Key altered biological processes included cell-cell communication (54%), deregulated nutrient sensing (39%), and loss of proteostasis (35%).
  • DEPs were significantly associated with neurological disorders such as Alzheimer's disease and schizophrenia.
  • Protein co-expression modules remained largely conserved across age groups, indicating functional homeostasis.

Conclusions:

  • This study provides a comprehensive proteomic profile of aging in the human OFC layer 2/3.
  • Age-related changes in the OFC involve critical cellular functions and are linked to neurological disease.
  • The conservation of protein co-expression modules suggests underlying homeostatic mechanisms that maintain brain function during aging.