Related Experiment Video

Updated: Feb 25, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

3.8K

Angiopoietin-like 4 directs uptake of dietary fat away from adipose during fasting

Emily M Cushing1, Xun Chi1, Kelli L Sylvers1

  • 1Department of Biochemistry, Fraternal Order of Eagles Diabetes Research Center, Obesity Research and Education Initiative, University of Iowa Carver College of Medicine, 169 Newton Rd, Iowa City, IA 52242, United States.

Molecular Metabolism
|July 29, 2017
PubMed
Abstract

Insights

Angiopoietin-like 4 (ANGPTL4) is induced during fasting to inhibit lipoprotein lipase (LPL). This process reduces triglyceride delivery to adipose tissue, aiding nutrient deprivation adaptation.

Area of Science:

  • Metabolism
  • Endocrinology
  • Molecular Biology

Background:

  • Angiopoietin-like 4 (ANGPTL4) regulates plasma triglyceride metabolism.
  • ANGPTL4 inhibits lipoprotein lipase (LPL), an enzyme crucial for triglyceride hydrolysis.

Purpose of the Study:

  • To investigate the induction kinetics of Angptl4 during fasting.
  • To test if ANGPTL4 physiologically reduces triglyceride delivery to adipose tissue during nutrient deprivation.

Main Methods:

  • Examined gene expression, LPL activity, and triglyceride uptake in mice.
  • Compared fasted and fed wild-type and Angptl4-deficient mice.

Main Results:

  • Angptl4 expression was rapidly induced early in fasting.
  • Fasting-induced Angptl4 expression was suppressed by food availability.
  • Angptl4-deficient mice showed increased LPL activity and adipose triglyceride uptake when fasted.

Conclusions:

  • Angptl4 is induced early in fasting.
  • This induction diverts fatty acid and triglyceride uptake away from adipose tissues.
Keywords:
ANGPTL4, angiopoietin-like 4BAT, brown adipose tissueGPIHBP1, glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1LPL, lipoprotein lipaseLipase inhibitionLipolysisLipoprotein metabolismPlasma triglyceridesgWAT, gonadal white adipose tissuemWAT, mesenchymal white adipose tissuesWAT, subcutaneous white adipose tissue

More Related Videos

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

3.0K
Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.8K

Related Concept Videos

Regulation of Food Intake01:30

Regulation of Food Intake

Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...
3.0K
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview
112.2K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.8K
Lipid Absorption01:24

Lipid Absorption

Dietary triglycerides from chyme in the duodenum are mixed with bile salts produced by the liver to emulsify fats. As a result, large droplets are broken down into smaller ones, increasing the surface area for enzymatic action. Once emulsified, pancreatic lipases hydrolyze the triglycerides into free fatty acids and monoglycerides.
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
3.1K