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Updated: Feb 25, 2026

Lung Fixation under Constant Pressure for Evaluation of Emphysema in Mice

Lung Fixation under Constant Pressure for Evaluation of Emphysema in Mice

Published on: September 26, 2019

Elastase-Induced Lung Emphysema Models in Mice.

Béla Suki¹, Erzsébet Bartolák-Suki², Patricia R M Rocco³

¹Department of Biomedical Engineering, Boston University, Boston, MA, USA. bsuki@bu.edu.

Methods in Molecular Biology (Clifton, N.J.)

|July 29, 2017

Summary

This summary is machine-generated.

Researchers detail two elastase-induced mouse models for studying pulmonary emphysema, a form of chronic obstructive pulmonary disease (COPD). These models help investigate disease progression and potential treatments when a cure is unavailable.

Keywords:

Airspace enlargement Anesthesia Compliance Porcine pancreatic elastase

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Area of Science:

Pulmonary Medicine
Animal Models
Pathology

Background:

Pulmonary emphysema, a form of chronic obstructive pulmonary disease (COPD), involves alveolar tissue destruction, reduced lung recoil, and breathing difficulties.
Currently, no cure exists for emphysema, necessitating the use of animal models to study its pathogenesis and progression.

Purpose of the Study:

To describe two distinct methods for inducing pulmonary emphysema in mice using elastase treatment.
To provide detailed protocols for these elastase-induced emphysema models in mice.

Main Methods:

Method 1: Single-dose oropharyngeal administration of elastase, followed by lung structure and function analysis between 2 days and 4 weeks post-treatment.
Method 2: Repeated (four times) intratracheal elastase administration, with observations 1-4 weeks after the final dose.

Main Results:

Detailed protocols for both single-dose and repeated-dose elastase-induced emphysema models are provided.
Examples illustrating lung structure and function in the emphysematous mouse model are included.

Conclusions:

Elastase-induced mouse models offer valuable tools for understanding emphysema pathogenesis and progression.
These established protocols facilitate research into potential therapeutic strategies for emphysema.