Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Selective sugar transport supports Proteus mirabilis fitness in the urinary tract.

PLoS pathogens·2026

Same author

Characterizing treatment initiation with central nervous system-active polypharmacy among adults with dementia.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026

Same author

Prediction of avatrombopag-induced thrombocytosis in pediatric immune thrombocytopenia: an AI-based real-world study.

Annals of hematology·2026

Same author

Consistency of a Dementia Diagnosis Between Systems for Patients Receiving Care Through the VA, Traditional Medicare, and Medicare Advantage.

Medical care·2026

Same author

Layered double hydroxide nanoplatform synergizes with sonodynamic therapy to induce dual activation of cuproptosis and ferroptosis for breast cancer treatment.

Bioorganic chemistry·2026

Same author

Rituximab-first versus eltrombopag monotherapy in pediatric persistent/chronic immune refractory thrombocytopenia after first-line therapy: a prospective multicenter cohort study.

Research and practice in thrombosis and haemostasis·2025

Search research articles

Related Experiment Video

Updated: Feb 25, 2026

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

Comparison of two decellularized dermal equivalents.

Shiuhyang Kuo¹, Hyungjin Myra Kim², Zhifa Wang³

¹Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.

Journal of Tissue Engineering and Regenerative Medicine

|July 29, 2017

Summary

Acellular dermal scaffolds like AlloDerm® and Allopatch support epithelium growth for tissue engineering. Allopatch showed superior blood vessel ingrowth, while AlloDerm® demonstrated faster cell migration in oral mucosa equivalents.

Keywords:

3D culture acellular dermal equivalents keratinocytes mucocutaneous junction oral mucosa scaffold skin tissue engineering

More Related Videos

Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

Published on: May 31, 2018

Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

Related Experiment Videos

Last Updated: Feb 25, 2026

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

Two Methods for Decellularization of Plant Tissues for Tissue Engineering Applications

Published on: May 31, 2018

Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

Area of Science:

Biomaterials Science
Tissue Engineering
Regenerative Medicine

Background:

Acellular dermal scaffolds offer a native-like matrix for tissue engineering, superior to synthetic options.
Variations in scaffold properties impact tissue-engineered graft outcomes.
Understanding scaffold characteristics is crucial for successful graft development.

Purpose of the Study:

To evaluate cellular growth and vascularization of AlloDerm® and Allopatch scaffolds for oral mucosa equivalents.
To compare the biological performance of two human cadaver-derived acellular dermal scaffolds.

Main Methods:

Fabrication of ex vivo produced oral mucosa equivalents (EVPOMEs) using primary human oral keratinocytes on AlloDerm® and Allopatch.
Implantation of EVPOMEs into immunodeficient mice to assess vascular ingrowth.
Co-culture keratinocyte models to evaluate cell migration rates on scaffolds.

Main Results:

Both AlloDerm® and Allopatch supported the development of a well-stratified epithelium.
Allopatch EVPOMEs exhibited 3.3 times higher blood vessel counts compared to AlloDerm® EVPOMEs (p=0.01).
Cell migration was slower on Allopatch compared to AlloDerm®.

Conclusions:

Both AlloDerm® and Allopatch are viable scaffolds for creating oral mucosa equivalents.
Allopatch promotes greater vascularization, while AlloDerm® facilitates faster cell migration.
Scaffold selection should consider specific tissue engineering goals, balancing vascularization and cell migration needs.