3-Iodothyronamine reduces insulin secretion in vitro via a mitochondrial mechanism

3-iodothyronamine (3-T₁AM), a decarboxylated and deiodinated thyroid hormone metabolite, leads at pharmacological doses to hypoinsulinemia, hyperglucagonemia and hyperglycemia in vivo. As the pancreatic Langerhans islets express thyroid hormone transmembrane transporters (THTT), we tested the hypothesis that not only plasma membrane-mediated 3-T₁AM binding to and activation of G-protein coupled receptors, but also 3-T₁AM metabolite(s) generated by 3-T₁AM uptake and metabolism might decrease glucose-stimulated insulin secretion (GSIS).

Murine pancreatic β-cells MIN6 were characterized for gene expression of THTT, deiodinases and monoamine oxidases. 3-T₁AM uptake and intracellular metabolism to the corresponding 3-iodothyroacetic acid were analysed by liquid-chromatography tandem mass spectrometry (LC-MS/MS) at different time points in cells as well as the conditioned medium. Mitochondrial activity, especially ATP-production, was monitored real-time after 3-T₁AM application using Seahorse Bioanalyzer technique. Effect of 3-T₁AM on GSIS into the culture medium was assayed by ELISA.

MIN6 cells express classical THTT, proposed to transport 3-T₁AM, as well as 3-T₁AM metabolizing enzymes comparable to murine primary pancreatic islets. 3-T₁AM accumulates in MIN6 cells and is metabolized by intracellular MaoB to 3-iodothyroacetic, which in turn is rapidly exported. 3-T₁AM decreases mitochondrial ATP-production concentration dependently. GSIS is diminished by 3-T₁AM treatment. Using LC-MS/MS, no further 3-T₁AM metabolites except 3-iodothyroacetic were detectable.

This data provides a first link between cellular 3-T₁AM uptake and regulation of mitochondrial energy metabolism in ß-cells, resulting in reduced insulin secretion. We conclude that MIN6 is an appropriate cell model to study 3-T₁AM-dependent (intra-)cellular biochemical mechanisms affecting insulin production in vitro.