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Updated: Feb 25, 2026
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
Published on: March 16, 2017
Construction of a ratiometric fluorescent probe with an extremely large emission shift for imaging hypochlorite in
Xuezhen Song1, Baoli Dong1, Xiuqi Kong1
1Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Shandong 250022, PR China.
Abstract:
Hypochlorite is one of the important reactive oxygen species (ROS) and plays critical roles in many biologically vital processes. Herein, we present a unique ratiometric fluorescent probe (CBP) with an extremely large emission shift for detecting hypochlorite in living cells. Utilizing positively charged α,β-unsaturated carbonyl group as the reaction site, the probe CBP itself exhibited near-infrared (NIR) fluorescence at 662nm, and can display strong blue fluorescence at 456nm when responded to hypochlorite. Notably, the extremely large emission shift of 206nm could enable the precise measurement of the fluorescence peak intensities and ratios. CBP showed high sensitivity, excellent selectivity, desirable performance at physiological pH, and low cytotoxicity. The bioimaging experiments demonstrate the biological application of CBP for the ratiometric imaging of hypochlorite in living cells.
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