Related Experiment Video

Updated: Aug 15, 2026

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
10:05

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

Published on: September 30, 2018

Processing of pro-colipase and trypsinogen by pancreatic dipeptidyl peptidase IV

E Heymann, R Mentlein, I Nausch

    Biomedica Biochimica Acta
    |January 1, 1986
    PubMed

    More Related Videos

    Pancreatic Tissue Dissection to Isolate Viable Single Cells08:04

    Pancreatic Tissue Dissection to Isolate Viable Single Cells

    Published on: May 26, 2023

    Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation07:44

    Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation

    Published on: April 3, 2026

    Related Concept Videos

    Lipid Digestion01:06

    Lipid Digestion

    Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
    Protein Digestion01:02

    Protein Digestion

    Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
    Insulin Secretory Vesicles01:05

    Insulin Secretory Vesicles

    Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
    Production of Pharmaceuticals01:30

    Production of Pharmaceuticals

    Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...
    Acute Pancreatitis II: Pathophysiology01:21

    Acute Pancreatitis II: Pathophysiology

    The pathophysiology of acute pancreatitis centers on injury to pancreatic acinar cells, which initiates a cascade of harmful intracellular events.This injury leads to premature activation of trypsinogen to trypsin in the pancreas. Trypsin then activates other digestive enzymes, such as chymotrypsin, elastase, and phospholipase A2, which begin breaking down pancreatic tissue. The resulting autodigestion causes local inflammation, tissue swelling, hemorrhage, and fat necrosis.Injured acinar cells...