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Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes
Yuka Unno1, Yoshinori Sato1, Satoshi Nishida1
1Department of Microbiology and Immunology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi, Tokyo 173-8605, Japan.
Abstract:
Acinetobacter baumannii is one of the most important nosocomial opportunistic pathogen worldwide. In addition, obesity has been associated with an increased risk of nosocomial infection, suggesting that there may be an association between A. baumannii and white adipose tissue. However, the effects of A. baumannii on adipocytes have not been well studied at the molecular level. Here, we investigated the potential role of A. baumannii-derived lipopolysaccharides (LPS) as signaling molecules that affect adipocyte functionality. We tested the effect of increasing concentrations of A. baumannii-derived LPS (10, 100, or 1000 ng/mL) on the 3T3-L1 adipocyte cell line. Exposure to LPS was found to increase the expression of several adipokines (e.g., MIP-2, MCP-1, TNF-α, IL-6, lipocalin-2, and FABP4) in 3T3-L1 adipocytes and significantly reduced the expression of leptin and adiponectin. The effects of A. baumannii-derived LPS on MIP-2 expression were similar in comparison with that of LPS prepared from Pseudomonas aeruginosa and Escherichia coli in our cell culture-based system. This study suggests that A. baumannii-derived LPS functions as a signaling molecule that impacts the inflammatory function of white adipose tissue on the level of gene expression.
Insights
Acinetobacter baumannii lipopolysaccharides (LPS) alter white adipose tissue gene expression, increasing inflammatory adipokines and decreasing leptin and adiponectin. This reveals LPS as a signaling molecule impacting adipocyte inflammatory function.
Area of Science:
- Microbiology
- Immunology
- Cell Biology
Background:
- Acinetobacter baumannii is a significant nosocomial pathogen.
- Obesity correlates with increased nosocomial infection risk, hinting at a link with adipose tissue.
- The molecular-level impact of A. baumannii on adipocytes remains underexplored.
Purpose of the Study:
- To investigate the role of A. baumannii-derived lipopolysaccharides (LPS) as signaling molecules affecting adipocyte function.
- To analyze the molecular effects of A. baumannii LPS on 3T3-L1 adipocytes.
Main Methods:
- Exposure of 3T3-L1 adipocytes to varying concentrations of A. baumannii-derived LPS (10, 100, 1000 ng/mL).
- Gene expression analysis of adipokines and other relevant markers.
Main Results:
- A. baumannii LPS significantly increased the expression of inflammatory adipokines (MIP-2, MCP-1, TNF-α, IL-6, lipocalin-2, FABP4).
- Leptin and adiponectin expression were significantly reduced following LPS exposure.
- The effect on MIP-2 expression was comparable to LPS from Pseudomonas aeruginosa and Escherichia coli.
Conclusions:
- A. baumannii-derived LPS acts as a signaling molecule influencing the inflammatory function of white adipose tissue at the gene expression level.
- These findings highlight a potential mechanism linking bacterial infection and metabolic dysfunction in adipose tissue.
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