Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Dolashki et al. Antimicrobial Activities of Different Fractions from Mucus of the Garden Snail <i>Cornu aspersum</i>. <i>Biomedicines</i> 2020, <i>8</i>, 315.

Biomedicines·2025
Same author

Comparison of Meglumine Antimoniate and Miltefosine in Cutaneous Leishmaniasis.

Journal of the College of Physicians and Surgeons--Pakistan : JCPSP·2023
Same author

Pecha Kucha in medical education: Promoting self-directed learning.

Medical education·2023
Same author

Selective Cytotoxicity and Changes in Protein Expression of T24 Bladder Carcinoma Permanent Cell Line after Treatment with Hemocyanins.

Current medicinal chemistry·2022
Same author

Quantitative proteomics to study aging in rabbit spleen tissues.

Experimental gerontology·2022
Same author

Effect and Mechanisms of Antibacterial Peptide Fraction from Mucus of <i>C. aspersum</i> against <i>Escherichia coli</i> NBIMCC 8785.

Biomedicines·2022
Same journal

Phytochemicals of Pteridophytes: Occurrence, Structures, and Biological Activity.

Progress in the chemistry of organic natural products·2026
Same journal

The Diels-Alder Reaction as a Key Step in Natural Product Synthesis.

Progress in the chemistry of organic natural products·2026
Same journal

Australian Rainforests-A Living Museum of Natural Product Chemical Diversity.

Progress in the chemistry of organic natural products·2025
Same journal

Biologically Active Compounds of Malaysian Medicinal and Aromatic Plants.

Progress in the chemistry of organic natural products·2025
Same journal

Phytochemistry of Bryophytes: Biologically Active Compounds and Their Uses as Cosmetics, Foods, and in Drug Development.

Progress in the chemistry of organic natural products·2025
Same journal

Tigliane Diterpenoids.

Progress in the chemistry of organic natural products·2024
See all related articles

Related Experiment Video

Updated: Feb 25, 2026

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

709

Human Deiminases: Isoforms, Substrate Specificities, Kinetics, and Detection.

Bushra Amin1, Wolfgang Voelter2

  • 1Department of Chemistry, University of Pittsburgh, Pittsburgh, 15260, PA, USA. bua4@pitt.edu.

Progress in the Chemistry of Organic Natural Products
|August 2, 2017
PubMed
Summary
This summary is machine-generated.

Peptidylarginine deiminase (PAD) enzymes convert arginine to citrulline, impacting protein function. Understanding PAD enzymes is crucial for autoimmune diseases, cancers, and demyelinating disorders.

Keywords:
Auto-citrullinationAutoimmune diseasesDetection assayEnzyme regulationNeurodegenerationPeptidylarginine deiminase (PAD) enzymesSelective inhibitorsSubstrate selectivityTherapeutic target

More Related Videos

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

10.2K
Author Spotlight: Advancing Cellular and Protein Engineering to Control Biological Functions and Develop Novel Therapies
04:17

Author Spotlight: Advancing Cellular and Protein Engineering to Control Biological Functions and Develop Novel Therapies

Published on: September 27, 2024

1.2K

Related Experiment Videos

Last Updated: Feb 25, 2026

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

709
Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

10.2K
Author Spotlight: Advancing Cellular and Protein Engineering to Control Biological Functions and Develop Novel Therapies
04:17

Author Spotlight: Advancing Cellular and Protein Engineering to Control Biological Functions and Develop Novel Therapies

Published on: September 27, 2024

1.2K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Peptidylarginine deiminase (PAD) enzymes catalyze arginine citrullination, altering protein structure and function.
  • Citrullination is implicated in multiple sclerosis pathogenesis and rheumatoid arthritis.
  • Autoantibodies to citrullinated proteins are diagnostic markers for rheumatoid arthritis.

Purpose of the Study:

  • To comprehensively survey the mechanism, kinetics, substrate selection, and specificities of PAD isoforms.
  • To improve understanding of PAD enzyme roles in demyelinating disorders, cancers, and autoimmune diseases.
  • To elucidate the regulation and biological/pathological effects of PAD enzymes.

Main Methods:

  • Literature review and synthesis of existing research on PAD enzymes.
  • Analysis of biochemical and physiological aspects of PAD enzyme activity.
  • Exploration of the role of PAD enzymes in various disease contexts.

Main Results:

  • PAD enzymes' citrullination activity critically influences protein structure and function.
  • Hyper-citrullination in multiple sclerosis contributes to demyelination and auto-antibody production.
  • Citrullinated protein autoantibodies are early diagnostic markers for rheumatoid arthritis.

Conclusions:

  • Further research into PAD enzyme mechanisms and regulation is essential for understanding their roles in disease.
  • PAD enzymes are significant targets for therapeutic interventions in autoimmune and demyelinating diseases.
  • A deeper understanding of PAD isoforms will advance diagnostics and treatments for various pathologies.