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Updated: Feb 25, 2026

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
Published on: April 19, 2021
The use of two-dimensional NMR relaxometry in bread staling: a valuable tool?
E Curti1, E Carini1, M F Cobo2
1Siteia.Parma Interdepartmental Centre, University of Parma, Parco Area Delle Scienze 181/a, 43124 Parma, Italy; Department of Food and Drug, University of Parma, Parco Area Delle Scienze 47/a, 43124 Parma, Italy.
Abstract:
Staling of bread was investigated in terms of physico-chemical parameters and one (1D) and two dimensional (2D) 1H Nuclear Magnetic Resonance (NMR) relaxometry. Physico-chemical properties were consistent with those generally reported for bread staling (crumb moisture loss, decrease in frozen water content, formation of amylopectin crystals, crumb hardening). One dimensional 1H NMR investigation suggested the presence of only one T1 protons population, while T2 was representative of multiple proton populations, that well related to the observed physico-chemical changes. 1H Two dimensional NMR provided an interesting insight of 1H T1 dynamics, as it allowed to discriminate the contribution of five protons pools within the 1H T1 relaxation.
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