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Updated: Feb 25, 2026

Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis
Published on: January 8, 2010
A revised method for the preparation of dry bone samples used in histological examination: Five simple steps
J G García-Donas1, A Dalton1, I Chaplin2
1Edinburgh Unit for Forensic Anthropology, School of History, Classics and Archaeology, Archaeology Department, University of Edinburgh, Edinburgh EH8 9AG, Scotland, UK.
Abstract:
Histology of dry bone tissue has many scientific applications. The histological analysis of bone requires the production of good quality thin sections. Many researchers have developed new histological techniques and/or they have refined existing ones. In this paper, we describe a revision of histological techniques for obtaining thin sections from modern dry bone. The method is easy to apply and the equipment required is commonly found in a histology laboratory. In comparison to other techniques presented in the literature, this adapted method reduces the number of consumables and steps, thereby improving the efficiency and cost-effectiveness of the procedure.
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