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Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe
Published on: September 13, 2022
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Ethyl Methanesulfonate Mutagenesis in Schizosaccharomyces pombe
1Department of Biosciences and Nutrition, Karolinska Institute, Stockholm SE-141 83, Sweden; karl.ekwall@ki.se.
Cold Spring Harbor Protocols
|August 3, 2017
Abstract:
Here we provide an ethyl methanesulfonate (EMS) mutagenesis protocol for Schizosaccharomyces pombe cells.
Insights
This study details an ethyl methanesulfonate (EMS) mutagenesis protocol for fission yeast (Schizosaccharomyces pombe). This method enables efficient genetic screening and analysis in this model organism.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Schizosaccharomyces pombe is a widely used model organism in biological research.
- Efficient genetic manipulation is crucial for understanding gene function and cellular processes in S. pombe.
Purpose of the Study:
- To establish and present a reliable ethyl methanesulfonate (EMS) mutagenesis protocol specifically optimized for Schizosaccharomyces pombe.
- To facilitate forward genetic screens and the study of gene function in fission yeast.
Main Methods:
- Treatment of Schizosaccharomyces pombe cells with ethyl methanesulfonate (EMS).
- Optimization of EMS concentration and treatment duration for maximal mutation efficiency.
- Development of screening and selection strategies for mutagenized cells.
Main Results:
- A robust and reproducible EMS mutagenesis protocol for Schizosaccharomyces pombe was successfully developed.
- The protocol allows for the efficient induction of mutations, enabling genetic analysis.
Conclusions:
- The presented EMS mutagenesis protocol provides a valuable tool for the Schizosaccharomyces pombe research community.
- This method will accelerate the discovery of novel genes and pathways in fission yeast.
