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Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying.

Mozhdeh Imaninezhad1, Era Jain2, Silviya Petrova Zustiak3,4

  • 1Department of Biomedical Engineering, Saint Louis University, St. Louis, MO, 63103, USA.

Methods in Molecular Biology (Clifton, N.J.)
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Summary

Electrohydrodynamic spraying (EHS) fabricates cell-laden polyethylene glycol (PEG) microspheres for biomedical uses. This method ensures high cell viability and efficiency in microencapsulation, offering a promising technique for tissue regeneration and cell delivery.

Keywords:
Cell microencapsulationElectrosprayingHydrogelsMicrospheresPolyethylene glycol (PEG)

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Area of Science:

  • Biomaterials Engineering
  • Cell Encapsulation Technologies
  • Regenerative Medicine

Background:

  • Cell microencapsulation is crucial for tissue regeneration and cell delivery applications.
  • Electrohydrodynamic spraying (EHS) is a promising technique for fabricating cell-laden hydrogel microspheres.
  • Existing methods may have limitations in terms of size control, throughput, or cell viability.

Purpose of the Study:

  • To describe an electrohydrodynamic spraying (EHS) technique for fabricating cell-laden polyethylene glycol (PEG) microspheres.
  • To optimize EHS parameters for efficient cell microencapsulation with high cell viability.
  • To demonstrate the impact of EHS parameters on microsphere characteristics and cell viability.

Main Methods:

  • Utilized electrohydrodynamic spraying (EHS) for microsphere fabrication.
  • Employed mild hydrogel gelation chemistry suitable for cell encapsulation.
  • Investigated the effect of EHS parameters: needle inner diameter, voltage, and flow rate.

Main Results:

  • Successfully fabricated cell-laden polyethylene glycol (PEG) microspheres using EHS.
  • Achieved high efficiency and viability for encapsulated cells.
  • Demonstrated that EHS parameters significantly influence microsphere size and cell viability.

Conclusions:

  • Electrohydrodynamic spraying (EHS) is an effective method for producing cell-laden PEG microspheres with high cell viability.
  • The EHS technique offers control over microsphere size and cell viability through parameter optimization.
  • This method holds potential for advancing cell-based therapies in tissue regeneration and drug delivery.