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Updated: Feb 25, 2026

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
Published on: October 2, 2018
Analysis of the dehydration process of caffeine using backscattering and transmission Raman spectroscopy
Rina Takeshima1, Yusuke Hattori2, Satoshi Managaki1
1Faculty of Technology, Musashino University, 3-3-3, Ariake, Koto-Ku, Tokyo 135-8181, Japan.
Abstract:
In the present study, the dehydration process of caffeine hydrate (CAH) was investigated by calibrating the moisture content in the caffeine tablet using backscattering Raman spectroscopy (BRS) and transmission Raman spectroscopy (TRS). The detectable depth of BRS is limited by its shallow laser penetration, while TRS is a powerful tool to determine the content of active pharmaceutical ingredients in a tablet. Our results suggest that the accuracy of a TRS-based calibration model falls beyond that of a BRS-based model. Based on the calibration used, the model was built by calculating the differences in the crystalline structures between hydrate and anhydrous caffeine. Moreover, it was demonstrated that the dehydration process occurred by switching water molecules between hydration sites of caffeine.
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