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Related Experiment Videos

Delayed type hypersensitivity induced by LPF from Bordetella pertussis.

J M Guadalupe Cortés, Y Juliano, N F Mendes

    Allergologia Et Immunopathologia
    |July 1, 1986
    PubMed
    Summary

    Lymphocytosis Promoting Factor (LPF) from Bordetella pertussis induces delayed hypersensitivity reactions similar to conventional antigens in humans and animals. This factor shows potential for assessing cell-mediated immunity without adverse effects.

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    Area of Science:

    • Immunology
    • Microbiology

    Background:

    • Lymphocytosis Promoting Factor (LPF) from Bordetella pertussis is known to induce lymphocytosis and lymphocyte blastogenesis.
    • LPF administration via intracutaneous injection elicits inflammatory reactions resembling delayed hypersensitivity.

    Purpose of the Study:

    • To evaluate the capacity of LPF to induce delayed hypersensitivity skin reactions in humans and animals.
    • To compare LPF-induced reactions with those of conventional microbial antigens used for cell-mediated immunity assessment.
    • To investigate the immunological mechanisms underlying LPF-induced delayed cutaneous hypersensitivity.

    Main Methods:

    • Intracutaneous injection of LPF and conventional microbial antigens in 35 cancer patients undergoing immunotherapy and 15 healthy individuals.
    • Histological examination of skin reactions in mice following LPF injections.

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  • Comparative analysis of cutaneous reactions to LPF and standard antigens.

    • Main Results:

    • LPF induced cutaneous reactions in humans and animals with characteristics of delayed hypersensitivity.
    • The reactions elicited by LPF were comparable to those induced by conventional antigens.
    • No adverse reactions were observed following intracutaneous LPF administration.

    Conclusions:

    • LPF can be utilized as a safe and effective agent for assessing cell-mediated immunity through delayed hypersensitivity skin testing.
    • LPF demonstrates potential as a diagnostic tool in immunological evaluations.
    • Further research is warranted to fully elucidate the immunological mechanisms involved.