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Manufacturing of Proteins and Antibodies: Chapter Downstream Processing Technologies
Richard Turner1, Adrian Joseph2, Nigel Titchener-Hooker2
1MedImmune LLC Gaithersburg Headquarters, One MedImmune Way, Gaithersburg, MD, 20878, USA.
Advances in Biochemical Engineering/Biotechnology
|August 5, 2017
Summary
Choosing the right cell harvesting method is crucial for bioprocess success. This review covers centrifugation and depth filtration, discussing optimization and scale-down strategies for efficient cell separation.
Area of Science:
- Biotechnology
- Bioprocess Engineering
- Cell Culture Technology
Background:
- Cell harvesting separates cells from the supernatant containing target molecules.
- Method selection depends on cell type, bioreactor operation, scale, and product/fluid characteristics.
- Traditional methods include filtration and centrifugation.
Purpose of the Study:
- To describe centrifugation and depth filtration harvesting methods.
- To share strategies for harvest optimization.
- To review alternative harvesting technologies and scale-down models.
Main Methods:
- Centrifugation
- Normal flow depth filtration
- Tangential flow microfiltration
Main Results:
- Predictable scale-down of harvest methods ensures successful production.
- Scale-down is critical for small-scale characterization studies.
- Recent developments in centrifugation scale-down models are presented.
Conclusions:
- Effective cell harvesting is vital for biopharmaceutical production.
- Optimization and understanding scale-down are key to successful bioprocesses.
- Review of alternative technologies provides a comprehensive overview.